Abstract

AND

Public Health Relevance

The purpose of the Core 2 is to assess the extent to which iPSC-derived neurons differentiated down a particular line Core 1 have achieved the expected mature phenotype. This is necessary so as to assure potential users of the properties of the neurons that are produced. The properties of such cells are extremely specific and the purpose of this core will be to examine how closely neurons produced from iPSC lines correspond to the known properties of the different neuronal types prior to handing them off to potential users for their individual projects. Core 2 will also be able to help clients answer specific questions about the status of different cellular characteristics in cells derived from different patient populations.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
1P30NS081774-01
Application #
8539211
Study Section
Special Emphasis Panel (ZNS1-SRB-R (59))
Project Start
Project End
2017-06-30
Budget Start
2012-09-30
Budget End
2013-06-30
Support Year
1
Fiscal Year
2012
Total Cost
$215,471
Indirect Cost
$60,601

  Institution

Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611

  Publications

Zunke, Friederike; Moise, Alexandra C; Belur, Nandkishore R et al. (2018) Reversible Conformational Conversion of ?-Synuclein into Toxic Assemblies by Glucosylceramide. Neuron 97:92-107.e10
Nguyen, Maria; Krainc, Dimitri (2018) LRRK2 phosphorylation of auxilin mediates synaptic defects in dopaminergic neurons from patients with Parkinson's disease. Proc Natl Acad Sci U S A 115:5576-5581
Huang, Tianzhi; Kim, Chung Kwon; Alvarez, Angel A et al. (2017) MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma. Cancer Cell 32:840-855.e8
Burbulla, Lena F; Song, Pingping; Mazzulli, Joseph R et al. (2017) Dopamine oxidation mediates mitochondrial and lysosomal dysfunction in Parkinson's disease. Science 357:1255-1261
Matsuoka, Akihiro J; Morrissey, Zachery D; Zhang, Chaoying et al. (2017) Directed Differentiation of Human Embryonic Stem Cells Toward Placode-Derived Spiral Ganglion-Like Sensory Neurons. Stem Cells Transl Med 6:923-936
Valdez, Clarissa; Wong, Yvette C; Schwake, Michael et al. (2017) Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients. Hum Mol Genet 26:4861-4872
Mazzulli, Joseph R; Zunke, Friederike; Tsunemi, Taiji et al. (2016) Activation of ?-Glucocerebrosidase Reduces Pathological ?-Synuclein and Restores Lysosomal Function in Parkinson's Patient Midbrain Neurons. J Neurosci 36:7693-706
Huang, Tianzhi; Alvarez, Angel A; Pangeni, Rajendra P et al. (2016) A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun 7:12885
Mazzulli, Joseph R; Zunke, Friederike; Isacson, Ole et al. (2016) ?-Synuclein-induced lysosomal dysfunction occurs through disruptions in protein trafficking in human midbrain synucleinopathy models. Proc Natl Acad Sci U S A 113:1931-6
Duan, Lishu; Peng, Chian-Yu; Pan, Liuliu et al. (2015) Human pluripotent stem cell-derived radial glia recapitulate developmental events and provide real-time access to cortical neurons and astrocytes. Stem Cells Transl Med 4:437-47

Showing the most recent 10 out of 13 publications