This proposal describes the establishment of a National Resource for Aplysia, whose overall goal is to provide consistently high quality cultured sea hares aplysia californica (and their cultured red algae food Gracilaria sp.) To NIH sponsored researchers. A. Californica is an important non- vertebrate (opisthobranch mollusc) model system for health related research, in the neurophysiology of behavior and learning. We will produce animals for research, and will conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-project/specific aims. 1. Production- We will increase our projected 1994 production by approximately 225% over the tenure of this grant and will make specimens from all life stages available at a price competitive with field-collected specimens. 2. Genetics- We will perform population genetic studies to characterize the amount of genetic variation present in our hatchery population in comparison to natural populations. The use of this genetic information will be to provide NIH-sponsored investigators with heterozygosity data for the hatchery population and with genetic markers for desirable/undesirable traits. 3. Developmental Neurophysiology-This project will investigate the regulation of neuronal excitability affecting reproductive development. The single cell voltage clamp technique will be applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in neurosecretory bag cells. Development of this modulatory capability is an integral part of sexual maturation. This sub-project will further characterize the neurophysiology of immature A. Californica, potentially leading to a new model use, and will yield information useful to hatchery maturation and breeding strategies. 4. Animal Health Monitoring-We will establish a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animal at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
Application #
Study Section
Special Emphasis Panel (CM)
Program Officer
Carrington, Jill L
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Miami Coral Gables
Other Domestic Higher Education
Coral Gables
United States
Zip Code
Hu, Jiangyuan; Adler, Kerry; Farah, Carole Abi et al. (2017) Cell-Specific PKM Isoforms Contribute to the Maintenance of Different Forms of Persistent Long-Term Synaptic Plasticity. J Neurosci 37:2746-2763
Hu, Jiangyuan; Ferguson, Larissa; Adler, Kerry et al. (2017) Selective Erasure of Distinct Forms of Long-Term Synaptic Plasticity Underlying Different Forms of Memory in the Same Postsynaptic Neuron. Curr Biol 27:1888-1899.e4
Jarvis, Tayler A; Capo, Thomas R; Bielmyer-Fraser, Gretchen K (2015) Dietary metal toxicity to the marine sea hare, Aplysia californica. Comp Biochem Physiol C Toxicol Pharmacol 174-175:54-64
Hu, Jiang-Yuan; Levine, Amir; Sung, Ying-Ju et al. (2015) cJun and CREB2 in the postsynaptic neuron contribute to persistent long-term facilitation at a behaviorally relevant synapse. J Neurosci 35:386-95
Hu, Jiangyuan; Schacher, Samuel (2015) Persistent Associative Plasticity at an Identified Synapse Underlying Classical Conditioning Becomes Labile with Short-Term Homosynaptic Activation. J Neurosci 35:16159-70
Hu, Jiang-Yuan; Schacher, Samuel (2014) Persistent long-term facilitation at an identified synapse becomes labile with activation of short-term heterosynaptic plasticity. J Neurosci 34:4776-85
Lee, Chang Young; Romanova, Elena V; Sweedler, Jonathan V (2013) Laminar stream of detergents for subcellular neurite damage in a microfluidic device: a simple tool for the study of neuroregeneration. J Neural Eng 10:036020
Ludwar, Bjoern Ch; Evans, Colin G; Cropper, Elizabeth C (2012) Monitoring changes in the intracellular calcium concentration and synaptic efficacy in the mollusc Aplysia. J Vis Exp :e3907
Albright, Rebecca; Bland, Charnelle; Gillette, Phillip et al. (2012) Juvenile growth of the tropical sea urchin Lytechinus variegatus exposed to near-future ocean acidification scenarios. J Exp Mar Bio Ecol 426-427:12-17
Romanova, Elena V; Sasaki, Kosei; Alexeeva, Vera et al. (2012) Urotensin II in invertebrates: from structure to function in Aplysia californica. PLoS One 7:e48764

Showing the most recent 10 out of 108 publications