High resolution images of molecular oxygenation images can provide crucial guides to the delivery and monitoring of cancer therapy. EPR imaging (EPRI) of oxygen provides a unique combination of spatial resolution, oxygen resolution, and uniform sensitivity with depth in tissue. This Center focuses on the optimization of In Vivo EPRI oxygen imaging anticipating human images. It is a consortium between the Universities of Chicago, Maryland and Denver. Successful images require coordinated development of instrumentation to obtain the images, spin probes to sample and report the tissue fluid environment and imaging strategies to optimally sample and analyze the information obtained. The Center is built on multidisciplinary effort from engineers, biologists, radiation oncologists, medical physicists, physical and organic chemists, statisticians and imaging mathematicians. In the past 21/2 years the Center 1) validated EPRI oxygen images point by point, 2) developed co-registration of oxygen with anatomy and other physiology, 3) increased animal tumor size imaged, 4) produced the first electron spin echo oxygen image nearly an order of magnitude faster than continuous wave (CW) acquisition, 5) developed a source of narrow line trityl spin probe capable of bulk synthesis, 6) synthesized persistent intracellular nitroxides and 7) radically altered reconstruction of images from projections allowing partial projections in even dimensions. These achievements directly improve spatial, oxygenation and/or temporal image resolution. Physiologically relevant resolution goals mandates improvement in resolution of EPRI oxygen images for small animals. This improvement will be necessary to scale to imaging larger volumes of tissue and tumor. We propose to: 1) Implement multiple techniques for CW acquisition to increase projection acquisition rate, select regions of interest and construct novel resonators 2) For pulsed image acquisition increase bandwidth and bandwidth uniformity as well as novel resonators and techniques to select regions of interest 3) Develop novel, even narrower line spin probes to with higher oxygen sensitivity 4) Build on the image acquisition and analysis progress. We hope to acquire a human image in the 5th year of support.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB002034-13
Application #
8293269
Study Section
Special Emphasis Panel (ZRG1-SBIB-K (40))
Program Officer
Sastre, Antonio
Project Start
1999-09-30
Project End
2013-05-31
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
13
Fiscal Year
2012
Total Cost
$1,000,099
Indirect Cost
$196,446
Name
University of Chicago
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Qiao, Zhiwei; Redler, Gage; Gui, Zhiguo et al. (2018) Three novel accurate pixel-driven projection methods for 2D CT and 3D EPR imaging. J Xray Sci Technol 26:83-102
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Kotecha, Mrignayani; Epel, Boris; Ravindran, Sriram et al. (2018) Noninvasive Absolute Electron Paramagnetic Resonance Oxygen Imaging for the Assessment of Tissue Graft Oxygenation. Tissue Eng Part C Methods 24:14-19
Qiao, Zhiwei; Redler, Gage; Qian, Yuhua et al. (2018) Investigation of the preconditioner-parameter in the preconditioned Chambolle-Pock algorithm applied to optimization-based image reconstruction. J Xray Sci Technol 26:435-448
Band, Alan; Donohue, Matthew P; Epel, Boris et al. (2018) Integration of a versatile bridge concept in a 34?GHz pulsed/CW EPR spectrometer. J Magn Reson 288:28-36
Kuzhelev, Andrey A; Tormyshev, Victor M; Rogozhnikova, Olga Yu et al. (2017) Triarylmethyl Radicals: EPR Study of 13C Hyperfine Coupling Constants. Z Phys Chem (N F) 231:777-794
Shi, Yilin; Quine, Richard W; Rinard, George A et al. (2017) Triarylmethyl Radical: EPR Signal to Noise at Frequencies between 250 MHz and 1.5 GHz and Dependence of Relaxation on Radical and Salt Concentration and on Frequency. Z Phys Chem (N F) 231:923-937
Boris, Epel; Sundramoorthy, Subramanian V; Halpern, Howard J (2017) 250 MHz passive Q-modulator for reflection resonators. Concepts Magn Reson Part B Magn Reson Eng 47B:
Epel, Boris; Sundramoorthy, Subramanian V; Krzykawska-Serda, Martyna et al. (2017) Imaging thiol redox status in murine tumors in vivo with rapid-scan electron paramagnetic resonance. J Magn Reson 276:31-36
Krzykawska-Serda, Martyna; Miller, Richard C; Elas, Martyna et al. (2017) Correlation Between Hypoxia Proteins and EPR-Detected Hypoxia in Tumors. Adv Exp Med Biol 977:319-325

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