Abstract

TRD 4: Next-generation nanoprobe toolkit for biomedical applications Investigators: Moungi Bawendi (MIT) (4.1), Ishan Barman (JHU) (4.2), Conor Evans (MGH) (4.2,4.3), Gabriela Schlau-Cohen (MIT) (4.3) Collaborative Projects: Rakesh Jain (MGH) (CP1), Saraswati Sukumar, Johns Hopkins (CP10); PnP Research Corporation(CP7), Matthew Coleman, UC Davis (CP9). This TRD adds a synergistic focus on the development of novel molecular probes to complement and enhance the strong spectroscopy, imaging hardware and label-free assay developments of the LBRC. Molecular probes, tailored to our hardware development projects provide powerful toolkits that meet the demands of our collaborators for ultrasensitive, gene- and protein-specific analysis. We seek to develop nanostructured probes that enable new modes of visualization of biological function in real time. We are pulled by our collaborators to develop engineered nanoprobes to study metastasis and treatment response (Jain, CP1), to understand epigenetics factors (Sukumar, CP10), and to optimize cancer therapeutics (Coleman, CP9; PnP Research Corporation, CP7). We will leverage our bright nanoparticles (NPs) in combination with our advances in the other TRDs to improve our understanding, diagnosis, and monitoring of critical pathological conditions. Ultimately, conjugation of these nanoprobes to targeting entities will enable facile detection of numerous orthogonal biological processes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB015871-34
Application #
9985832
Study Section
Special Emphasis Panel (ZEB1)
Project Start
1997-06-01
Project End
2022-05-31
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
34
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02142

  Publications

Jonas, Oliver; Kang, Jeon Woong; Singh, Surya P et al. (2018) In vivo detection of drug-induced apoptosis in tumors using Raman spectroscopy. Analyst 143:4836-4839
Bartelt, Alexander; Widenmaier, Scott B; Schlein, Christian et al. (2018) Brown adipose tissue thermogenic adaptation requires Nrf1-mediated proteasomal activity. Nat Med 24:292-303
Ahmad, Azeem; Dubey, Vishesh; Singh, Vijay Raj et al. (2018) Quantitative phase microscopy of red blood cells during planar trapping and propulsion. Lab Chip 18:3025-3036
Singh, Surya P; Mukherjee, Soumavo; Galindo, Luis H et al. (2018) Evaluation of accuracy dependence of Raman spectroscopic models on the ratio of calibration and validation points for non-invasive glucose sensing. Anal Bioanal Chem 410:6469-6475
Wadduwage, Dushan N; Kay, Jennifer; Singh, Vijay Raj et al. (2018) Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice. Sci Rep 8:12108
Zhang, Chi; Winnard Jr, Paul T; Dasari, Sidarth et al. (2018) Label-free Raman spectroscopy provides early determination and precise localization of breast cancer-colonized bone alterations. Chem Sci 9:743-753
Rizwan, Asif; Paidi, Santosh Kumar; Zheng, Chao et al. (2018) Mapping the genetic basis of breast microcalcifications and their role in metastasis. Sci Rep 8:11067
Xue, Yi; So, Peter T C (2018) Three-dimensional super-resolution high-throughput imaging by structured illumination STED microscopy. Opt Express 26:20920-20928
Pandey, Rishikesh; Singh, Surya P; Zhang, Chi et al. (2018) Label-free spectrochemical probe for determination of hemoglobin glycation in clinical blood samples. J Biophotonics 11:e201700397
Carr, Jessica A; Franke, Daniel; Caram, Justin R et al. (2018) Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. Proc Natl Acad Sci U S A 115:4465-4470

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