TR&D 2: Bioprinting Patterning for Cell-Laden Constructs Project Summary A major challenge for tissue and organ engineering is the production of three-dimensional (3D) biomimetic, cellular tissue constructs of clinically relevant size, shape, and structural integrity needed for the replacement of damaged or injured tissues. To address this need, we have been investigating 3D bioprinting technologies, which are designed to print cell-laden hydrogel bioinks as well as polymeric biomaterials, to manufacture complex, multi-cellular living tissue constructs that mimic the structure of native tissues. In addition, we have been optimizing the formulation of biomaterials to serve as the scaffolding for 3D bioprinting, and providing the biological environment needed for the successful delivery of cells and biomaterials to specific locations within the 3D structures. It has become evident that the patterning design of the cell-laden biomaterials is critical for achieving adequate nutrition and functional outcomes. In this project, we will (1) develop bioink formulations and functionalize to control cell positioning with high printability, (2) fabricate biofunctional bioink formulations to control the cell microenvironment, and (3) validate bioprinted multiple cell populations with interacting functionalities for bone tissue regeneration in vivo. The results of this project - which will be shared with the broad community - will be to establish the ideal parameters needed for cell positioning during 3D printing of tissues, thus establishing a community wide approach for engineering tissues.

Public Health Relevance

TR&D 2: Bioprinting Patterning for Cell-Laden Constructs Project Narrative A major challenge for tissue and organ engineering is the production of three-dimensional (3D) tissue constructs of clinically relevant size, shape, and structural integrity needed for the replacement of damaged or injured tissues. We developed a novel 3D bioprinting system that is designed to print living tissue constructs that mimic the structure of native tissues. We aim to develop optimized bioprinting parameters that could recapitulate tissue microenvironment to build multi-cellular living tissue constructs with interacting functionalities. Developing open source formulations for precise cell positioning and functionalized polymer printing would be beneficial for establishing a community wide approach for engineering tissues or organs.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB023833-03
Application #
9650580
Study Section
Special Emphasis Panel (ZEB1)
Project Start
Project End
Budget Start
2019-02-01
Budget End
2020-01-31
Support Year
3
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Maryland College Park
Department
Type
DUNS #
790934285
City
College Park
State
MD
Country
United States
Zip Code
20742
Patel, Divya B; Luthers, Christopher R; Lerman, Max J et al. (2018) Enhanced extracellular vesicle production and ethanol-mediated vascularization bioactivity via a 3D-printed scaffold-perfusion bioreactor system. Acta Biomater :
Kim, Soon Hee; Yeon, Yeung Kyu; Lee, Jung Min et al. (2018) Publisher Correction: Precisely printable and biocompatible silk fibroin bioink for digital light processing 3D printing. Nat Commun 9:2350
Bittner, Sean M; Guo, Jason L; Melchiorri, Anthony et al. (2018) Three-dimensional Printing of Multilayered Tissue Engineering Scaffolds. Mater Today (Kidlington) 21:861-874
Santoro, Marco; Navarro, Javier; Fisher, John P (2018) Micro- and Macrobioprinting: Current Trends in Tissue Modeling and Organ Fabrication. Small Methods 2:
Chim, Letitia K; Mikos, Antonios G (2018) Biomechanical forces in tissue engineered tumor models. Curr Opin Biomed Eng 6:42-50
Gao, Teng; Gillispie, Gregory J; Copus, Joshua S et al. (2018) Optimization of gelatin-alginate composite bioink printability using rheological parameters: a systematic approach. Biofabrication 10:034106
Tang, Qinggong; Piard, Charlotte; Lin, Jonathan et al. (2018) Imaging stem cell distribution, growth, migration, and differentiation in 3-D scaffolds for bone tissue engineering using mesoscopic fluorescence tomography. Biotechnol Bioeng 115:257-265
Smoak, Mollie M; Pearce, Hannah A; Mikos, Antonios G (2018) Microfluidic devices for disease modeling in muscle tissue. Biomaterials :
Guo, Ting; Ringel, Julia P; Lim, Casey G et al. (2018) Three dimensional extrusion printing induces polymer molecule alignment and cell organization within engineered cartilage. J Biomed Mater Res A 106:2190-2199
Guo, Ting; Noshin, Maeesha; Baker, Hannah B et al. (2018) 3D printed biofunctionalized scaffolds for microfracture repair of cartilage defects. Biomaterials 185:219-231

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