This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Synaptotagmins have been shown to regulate the mechanism of vesicle fusion. We are using immuno-EM tomography to study the localization of synaptotagmins within the chemical synapse. Because synaptotagmins regulate fusion mechanism we are particularly interested in the distribution of various synaptotagmin isoforms on synaptic vesicles. Our preliminary tomographic data have demonstrated a biased vesicle population, which matches a previously observed bias in vesicle fusion mechanism. Immuno-EM tomographic studies are ongoing in an effort to understand the relationship between vesicle morphology, synaptotagmin isoforms, and neurotransmitter packaging.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR000592-36
Application #
7355001
Study Section
Special Emphasis Panel (ZRG1-CB-J (40))
Project Start
2006-09-26
Project End
2007-07-31
Budget Start
2006-09-26
Budget End
2007-07-31
Support Year
36
Fiscal Year
2006
Total Cost
$9,371
Indirect Cost
Name
University of Colorado at Boulder
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
007431505
City
Boulder
State
CO
Country
United States
Zip Code
80309
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