This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Synaptotagmins have been shown to regulate the mechanism of vesicle fusion. We are using immuno-EM tomography to study the localization of synaptotagmins within the chemical synapse. Because synaptotagmins regulate fusion mechanism we are particularly interested in the distribution of various synaptotagmin isoforms on synaptic vesicles. Our preliminary tomographic data have demonstrated a biased vesicle population, which matches a previously observed bias in vesicle fusion mechanism. Immuno-EM tomographic studies are ongoing in an effort to understand the relationship between vesicle morphology, synaptotagmin isoforms, and neurotransmitter packaging.
Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017: |
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Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680 |
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Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222 |
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