As a model for understanding RNA-protein interactions, we are studying the structure of the Rev Response Element RNA in complex with peptides derived from the Rev protein. Rev is one of two small basic regulatory protein in HIV. Binding of Rev to the Rev-response element RNA results in a change in the ratio of spliceD and unspliced viral mRNAs that appear in the cytoplasm. Formation of this interaction is a key point in the replication cycle of HIV. In addition, we are also examining a model of the HIV TAR RNA in complex with argininamide and the base triple formed between an A-U base pair in the upper stem of HIV TAR RNA.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000995-25
Application #
6355172
Study Section
Project Start
2000-05-01
Project End
2001-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
25
Fiscal Year
2000
Total Cost
$42,845
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
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