This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Metal hyperaccumulating plants have the extraordinary ability to accumulate between 1-3% of their shoot dry weight as various metals including Ni, Zn and the nonmetal Se. Because of this ability the genetic material in these plants is valuable for the development of both efficient phytoremediation crop plants and foodstuffs enriched in essential trace elements such as Zn and Se. In order to determine the molecular basis of this hyperaccumulation phenotype, for the identification of these valuable genetic resources, we propose to perform an integrated series of both molecular, biochemical and x-ray experiments including both XANES and EXAFS. This work builds directly on our previous studies over the last seven years in this area.
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