This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.We propose to study solution structure of anthrax toxin protective antigen (PA) and lethal factor (LF) by solution x-ray scattering to complement our crystallographic studies. PA has a flexible loop which forms an attachment pore to host cell membrane, but the loop is invisible in our crystal structure. We wish to determine the low resolution structure of PA and visualize the loop by fitting the crystal structure into the solution structure. The pH-dependent pore formation of the PA loop will also be studied. We also plan on studying LF in solution to complement our crystallographic studies on the conformational change of LF essential for acquiring toxicity when PA and LF forms a complex. Finally, we wish to study the solution structure of PA-LF complex as very little is know about how LF and PA interact.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-28
Application #
7598141
Study Section
Special Emphasis Panel (ZRG1-BPC-E (40))
Project Start
2007-03-01
Project End
2008-02-29
Budget Start
2007-03-01
Budget End
2008-02-29
Support Year
28
Fiscal Year
2007
Total Cost
$2,977
Indirect Cost
Name
Stanford University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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