Abstract

Bioorienatation of chromosomes during prometaphase is believed to require an activity of two sister kinetochores placed on opposite sides of the chromosome. Recently, we have found that chromosomes can become biooriented and congress onto the metaphase plate even after one kinetochore has been destroyed by laser microirradiation.This indicates that a single kinetochore can interact with microtubles from two opposite poles. A 3-D reconstruction of the kinetochore region of the chromosome after laser irradiation will demonstrate whether microtubules from both opposing spindle poles terminate in the same (single) kinetochore.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001219-15
Application #
5222914
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248
Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7
Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78
Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63
Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12
Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12
McEwen, Bruce F; Dong, Yimin (2010) Contrasting models for kinetochore microtubule attachment in mammalian cells. Cell Mol Life Sci 67:2163-72
Palladino, Michael J (2010) Modeling mitochondrial encephalomyopathy in Drosophila. Neurobiol Dis 40:40-5
Lu, Zonghuan; McMahon, Jay; Mohamed, Hisham et al. (2010) Passive Microfluidic device for Sub Millisecond Mixing. Sens Actuators B Chem 144:301-309
Bernhard, Joan M; Goldstein, Susan T; Bowser, Samuel S (2010) An ectobiont-bearing foraminiferan, Bolivina pacifica, that inhabits microxic pore waters: cell-biological and paleoceanographic insights. Environ Microbiol 12:2107-19

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