(Supported in part by NSF DPP89-17375 to S. Bowser) Almost every cell in the human body contains a single primary cilium that extends from the mature centriole of the centrosome. In some tissues, e.g., kidney epithelia, the cilia projects many micrometers from the cellular surface. Because of their diminutive size (0.25lm thick) primary cilia are difficult to study in vivo and their function remains unknown. We have developed the first method for viewing primary cilia in living cells. It is based on growing kidney cells on Formvar films, and then folding these films between two coverslips. This allows individual living cells, and their associated primary cilia, to be examined along with length for considerable periods by video-enhanced DIC LM. Using this approach we are studying the material properties and motility associated with primary cilia. We are also using low-light level fluorescence LM, in conjunction with DIC, to examine surface chemistry and cytoplasmic exc hange within primary cilia. Same-cell correlative LM/HVEM is used to examine ultrastructural correlates of the LM findings.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001219-17
Application #
6280706
Study Section
Project Start
1998-01-01
Project End
1998-12-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
17
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Wadsworth Center
Department
Type
DUNS #
110521739
City
Menands
State
NY
Country
United States
Zip Code
12204
Booth, David M; Enyedi, Balázs; Geiszt, Miklós et al. (2016) Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface. Mol Cell 63:240-248
Mannella, Carmen A; Lederer, W Jonathan; Jafri, M Saleet (2013) The connection between inner membrane topology and mitochondrial function. J Mol Cell Cardiol 62:51-7
Takvorian, Peter M; Buttle, Karolyn F; Mankus, David et al. (2013) The multilayered interlaced network (MIN) in the sporoplasm of the microsporidium Anncaliia algerae is derived from Golgi. J Eukaryot Microbiol 60:166-78
Forbes, Stephen J; Martinelli, Daniel; Hsieh, Chyongere et al. (2012) Association of a protective monoclonal IgA with the O antigen of Salmonella enterica serovar Typhimurium impacts type 3 secretion and outer membrane integrity. Infect Immun 80:2454-63
Wang, Ruiwu; Zhong, Xiaowei; Meng, Xing et al. (2011) Localization of the dantrolene-binding sequence near the FK506-binding protein-binding site in the three-dimensional structure of the ryanodine receptor. J Biol Chem 286:12202-12
Marko, Michael; Leith, Ardean; Hsieh, Chyongere et al. (2011) Retrofit implementation of Zernike phase plate imaging for cryo-TEM. J Struct Biol 174:400-12
Lu, Zonghuan; McMahon, Jay; Mohamed, Hisham et al. (2010) Passive Microfluidic device for Sub Millisecond Mixing. Sens Actuators B Chem 144:301-309
Bernhard, Joan M; Goldstein, Susan T; Bowser, Samuel S (2010) An ectobiont-bearing foraminiferan, Bolivina pacifica, that inhabits microxic pore waters: cell-biological and paleoceanographic insights. Environ Microbiol 12:2107-19
Liu, Zheng; Wang, Ruiwu; Tian, Xixi et al. (2010) Dynamic, inter-subunit interactions between the N-terminal and central mutation regions of cardiac ryanodine receptor. J Cell Sci 123:1775-84
Springer, Deborah J; Ren, Ping; Raina, Ramesh et al. (2010) Extracellular fibrils of pathogenic yeast Cryptococcus gattii are important for ecological niche, murine virulence and human neutrophil interactions. PLoS One 5:e10978

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