The streptavidin-biotin system is being used as a model system to investigatewhether highly ordered surface monolayers can mitigate non-specific cell response to biomaterial surfaces, and whether specific cell responses can be engineered by incorporating appropriate peptide signals into such a surface. At present, ESCA and SIMS are being used to characterize streptavidin adsorbed onto biotin-terminated self-assembled monolayers. Eventually streptavidin derivatives having specific peptide sequences will be employed to study whether cell response can be controlled when these peptide sequences are exposed. Mixed derivative populations will later be investigated. Endothelial cells and smooth muscle cells will be used to determine cell response to the surfaces.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001296-14
Application #
6119922
Study Section
Project Start
1998-07-01
Project End
1999-09-27
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
14
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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