We are using fluorescence depolarization as an independent technique to ask why the solution NMR and X-ray diffraction structures of a RNA dodecamer grossly disagree (single-stranded loop vs. double helix). We demonstrated that this RNA tetraloop and several analogs maintain single-strand hairpin conformations in solution in the conditions used to grow the crystal. We have also used this technique to study base pair twisting in short DNA duplexes. These duplexes were modeled as cylinders, and rotational diffusion coefficients were determined.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001348-17
Application #
6281071
Study Section
Project Start
1998-08-01
Project End
1999-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
17
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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