This research program is studying the biophysical properties and subunit interactions of voltage gated K+ channels and their roles in the physiology of T Lymphocytes. Specifically, we are determining the physiological impact of irreversibly ablating specific endogenous K+ channel isoforms in primary human T cells on membrane potential, regulatory volume decrease, interleukin-2 secretion, cell proliferation and apoptosis (programmed cell death). Additionally, we are determining turnover rates of functional channels and K+ channel mRNA steady-state levels to assess T cell responses to elimination of K+ current. We have chosen to use a molecular laser ablation technique, chromophore laser assisted inactivation (CALI).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001348-17
Application #
6281072
Study Section
Project Start
1998-08-01
Project End
1999-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
17
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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