This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.
The aim of this project is to further develop and maintain state of the art fast and ultrafast methods of optical and infrared spectroscopy that are indispensable for the study of a wide range of biological processes stretched over the widest possible range of time scales. Part of the work is aiming at developing new techniques for excited state lifetime and anisotropy measurements that use spontaneous emission as the detection of the excited state. This method will be applied to determine the excited state properties of DNA probes in collaborative experiments with Prof. Burgess. It is also proposed to incorporate a NOPA device into the ultrafast fluorescence upconversion apparatus which would be particularly important in the dynamical analysis of the DNA-labeling cassettes. For example, in those studies, it would important to employ excitation wavelengths specifically tuned to the absorption maxima of the energy donor chromophores near 490 nm, a wavelength not available by simply using the second harmonic of a Ti:S output.
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