MacCHESS has implemented five CCD-based x-ray detectors; a Princeton 1k detector, the Princeton Scientific Instruments 2k detector, an Area Detector Systems Corporation (ADSC) 1k detector and two ADSC Quantum 4 mosaic detectors. As part of the process of testing and calibrating these detectors we routinely measure data using the various CCD detector systems. During the past year the two Quantum 4 detectors were installed and debugged and are now routinely available for routine data collection. As part of the installation, a detector mount was developed that provide for a two-theta offset. During the past year we have developed a local calibration system based on a portable x-ray generator. In addition, we found that the DENZO data processing package did not support the Quantum 4 detector and established a collaboration to develop a separate software package using MOSFLM as the integration program. All of these developments have reached a stage where users can routinely collect and process data. All of these developments were first tested using crystals for which we have a great deal of experience. These include bovine purine nucleoside phosphorylase (cubic) and E.coli purine nucleoside phosphorylase (hexagonal) which we routinely use for testing detector performance. Both crystals are reproducible, diffract to high resolution, freeze well and have high symmetry. Therefore, they provide excellent test samples for detector testing. In the future we will test a 2k CCD module from ADSC that will serve as the basis of a 2k version of the Quantum 4. This detector will have an active area of 20 cm x 20 cm and 16 million pixels.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
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Kozlov, Guennadi; Wong, Kathy; Gehring, Kalle (2018) Crystal structure of the Legionella effector Lem22. Proteins 86:263-267
Ménade, Marie; Kozlov, Guennadi; Trempe, Jean-François et al. (2018) Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations. J Biol Chem 293:12832-12842
Xu, Jie; Kozlov, Guennadi; McPherson, Peter S et al. (2018) A PH-like domain of the Rab12 guanine nucleotide exchange factor DENND3 binds actin and is required for autophagy. J Biol Chem 293:4566-4574
Dean, Dexter N; Rana, Pratip; Campbell, Ryan P et al. (2018) Propagation of an A? Dodecamer Strain Involves a Three-Step Mechanism and a Key Intermediate. Biophys J 114:539-549
Chen, Yu Seby; Kozlov, Guennadi; Fakih, Rayan et al. (2018) The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity. J Biol Chem 293:19998-20007
Xu, Caishuang; Kozlov, Guennadi; Wong, Kathy et al. (2016) Crystal Structure of the Salmonella Typhimurium Effector GtgE. PLoS One 11:e0166643
Cogliati, Massimo; Zani, Alberto; Rickerts, Volker et al. (2016) Multilocus sequence typing analysis reveals that Cryptococcus neoformans var. neoformans is a recombinant population. Fungal Genet Biol 87:22-9
Oot, Rebecca A; Kane, Patricia M; Berry, Edward A et al. (2016) Crystal structure of yeast V1-ATPase in the autoinhibited state. EMBO J 35:1694-706
Lucido, Michael J; Orlando, Benjamin J; Vecchio, Alex J et al. (2016) Crystal Structure of Aspirin-Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Reversed Stereochemistry. Biochemistry 55:1226-38
Bauman, Joseph D; Harrison, Jerry Joe E K; Arnold, Eddy (2016) Rapid experimental SAD phasing and hot-spot identification with halogenated fragments. IUCrJ 3:51-60

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