Abstract

Dr. Taegtmeyer's group continues to examine mobilization of glycogen and glycogen turnover during normoxia and myocardial ischemia. The goal is to use 13C NMR isotopomer analysis to monitor the contribution of glycogen to acetyl-CoA and energy generation. The basic protocol uses depletion followed by glycogen repletion with 13C enriched glycosyl units. Tissue extracts from experiments performed in Houston were analyzed at the Resource and demonstrated that lactate, glutamate and glucose could be easily detected. However, with the very brief (3 min) period for citric acid cycle turnover plus the low enrichment inherent in the use of [1-13C] glucose, 13C-13C spin-spin coupling could not be detected. These experiments were redesigned with [1,6-13C] glucose and a longer period for cycle turnover which should yield spectra suitable for analysis. (Collaborative 7) REPORT PERIOD: (09/01/97-08/31/98)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002584-13
Application #
6335259
Study Section
Project Start
2000-08-15
Project End
2001-08-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
2000
Total Cost
$6,953
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Chiu, Tsuicheng D; Arai, Tatsuya J; Campbell Iii, James et al. (2018) MR-CBCT image-guided system for radiotherapy of orthotopic rat prostate tumors. PLoS One 13:e0198065
Mishkovsky, Mor; Anderson, Brian; Karlsson, Magnus et al. (2017) Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized 13C magnetic resonance. Sci Rep 7:11719
Moreno, Karlos X; Harrison, Crystal E; Merritt, Matthew E et al. (2017) Hyperpolarized ?-[1-13 C]gluconolactone as a probe of the pentose phosphate pathway. NMR Biomed 30:
Funk, Alexander M; Anderson, Brian L; Wen, Xiaodong et al. (2017) The rate of lactate production from glucose in hearts is not altered by per-deuteration of glucose. J Magn Reson 284:86-93
Zhang, Liang; Habib, Amyn A; Zhao, Dawen (2016) Phosphatidylserine-targeted liposome for enhanced glioma-selective imaging. Oncotarget 7:38693-38706
Walker, Christopher M; Merritt, Matthew; Wang, Jian-Xiong et al. (2016) Use of a Multi-compartment Dynamic Single Enzyme Phantom for Studies of Hyperpolarized Magnetic Resonance Agents. J Vis Exp :e53607
Wu, Yunkou; Zhang, Shanrong; Soesbe, Todd C et al. (2016) pH imaging of mouse kidneys in vivo using a frequency-dependent paraCEST agent. Magn Reson Med 75:2432-41
Malloy, Craig R; Sherry, A Dean (2016) Biochemical Specificity in Human Cardiac Imaging by 13C Magnetic Resonance Imaging. Circ Res 119:1146-1148
Moss, Lacy R; Mulik, Rohit S; Van Treuren, Tim et al. (2016) Investigation into the distinct subcellular effects of docosahexaenoic acid loaded low-density lipoprotein nanoparticles in normal and malignant murine liver cells. Biochim Biophys Acta 1860:2363-2376
Bastiaansen, Jessica A M; Merritt, Matthew E; Comment, Arnaud (2016) Measuring changes in substrate utilization in the myocardium in response to fasting using hyperpolarized [1-(13)C]butyrate and [1-(13)C]pyruvate. Sci Rep 6:25573

Showing the most recent 10 out of 374 publications