The major drawback of pulsed two-photon excitation schemes in fluorescence correlation spectroscopy compared to one-photon applications is the existence of comparably long dark periods (12ns) for pulsed excitation, since the fluorescence lifetime is for most fluorophors only several ns. By splitting the laser beam and using different delay lines, we built a pulse doubler and quadrupler that was applied to FCS. The signal-to-noise ratio could be improved by a factor of 1.5 with proper alignment.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR004224-11
Application #
6121853
Study Section
Project Start
1998-09-30
Project End
1999-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
11
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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McMullen, Jesse D; Zipfel, Warren R (2010) A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection. Opt Express 18:5390-8

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