Crystallographic studies of weakly-diffracting biological samples using bending-magnet beamlines at the NSLS frequently are limited by the low intensity of the focussed, monochromatic X-ray beam striking the sample. For example, direct comparison of beamlines X4A, X8C X I 2B, and X I 2C (bending magnets) with beamline X25 (wiggler) demonstrated that the bending-magnet beamlines do not offer sufficient flux for studies of some of the most interesting and difficult crystallographic problems - large macromolecular complexes - at their highest possible resolution limits. With the growing number of protein crystallography laboratories in both academe and industry, wiggler beamlines suitable for routine x-ray diffraction data collection from the most weakly diffracting specimens are now heavily over- subscribed. Acknowledging the difficulty in simply building more wiggler beamlines, one would like to find an alternative means to provide dramatically more x-ray flux at the NSLS so that one ca n approach these difficult problems.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR012408-04
Application #
6346387
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
4
Fiscal Year
2000
Total Cost
$163,125
Indirect Cost
Name
Brookhaven National Laboratory
Department
Type
DUNS #
027579460
City
Upton
State
NY
Country
United States
Zip Code
11973
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