Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed Center will provide access to its existing methods as well as newly developed methods through a service component. The analytical services it will provide include a comprehensive set of methods for the analysis of monosaccharides using CE, polysaccharides cleaved from proteins using MS, and glycosylation of tryptic peptides using LC/MS methods. The services offered will also include standard proteomic analyses such as 2D gel electrophoresis, peptide fingerprinting for protein identification, and the analysis of post-translational protein modifications other than glycosylat

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR018942-02
Application #
7359157
Study Section
Special Emphasis Panel (ZRG1-BCMB-M (40))
Project Start
2006-09-01
Project End
2007-08-31
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
2
Fiscal Year
2006
Total Cost
$43,915
Indirect Cost

  Institution

Name
Indiana University Bloomington
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47401

  Publications

Yu, Chuan-Yih; Mayampurath, Anoop; Tang, Haixu (2013) Software tools for glycan profiling. Methods Mol Biol 951:269-76
Novotny, Milos V; Alley Jr, William R; Mann, Benjamin F (2013) Analytical glycobiology at high sensitivity: current approaches and directions. Glycoconj J 30:89-117
Balonova, Lucie; Mann, Benjamin F; Cerveny, Lukas et al. (2012) Characterization of protein glycosylation in Francisella tularensis subsp. holarctica: identification of a novel glycosylated lipoprotein required for virulence. Mol Cell Proteomics 11:M111.015016
Alley Jr, William R; Vasseur, Jacqueline A; Goetz, John A et al. (2012) N-linked glycan structures and their expressions change in the blood sera of ovarian cancer patients. J Proteome Res 11:2282-300
Isailovic, Dragan; Plasencia, Manolo D; Gaye, Maissa M et al. (2012) Delineating diseases by IMS-MS profiling of serum N-linked glycans. J Proteome Res 11:576-85
Mayampurath, Anoop M; Wu, Yin; Segu, Zaneer M et al. (2011) Improving confidence in detection and characterization of protein N-glycosylation sites and microheterogeneity. Rapid Commun Mass Spectrom 25:2007-19
Zhuang, Zexi; Mitra, Indranil; Hussein, Ahmed et al. (2011) Microchip electrophoresis of N-glycans on serpentine separation channels with asymmetrically tapered turns. Electrophoresis 32:246-53
Kang, Pilsoo; Madera, Milan; Alley Jr, William R et al. (2011) Glycomic Alterations in the Highly-abundant and Lesser-abundant Blood Serum Protein Fractions for Patients Diagnosed with Hepatocellular Carcinoma. Int J Mass Spectrom 305:185-198
Kurulugama, Ruwan T; Nachtigall, Fabiane M; Valentine, Stephen J et al. (2011) Overtone mobility spectrometry: part 4. OMS-OMS analyses of complex mixtures. J Am Soc Mass Spectrom 22:2049-60
Alley Jr, William R; Madera, Milan; Mechref, Yehia et al. (2010) Chip-based reversed-phase liquid chromatography-mass spectrometry of permethylated N-linked glycans: a potential methodology for cancer-biomarker discovery. Anal Chem 82:5095-106

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