Toxic chemicals have been accumulating in various waste sites as well as in other industrial, urban and rural areas throughout the United States. In this project, we plan to study basic mechanisms and toxicological meaning of microbial and photochemical degradation technologies to promote on-site (in situ) degradation of toxic wastes. We plan to utilize Phanerochaete chrysosporium, a well-studied white rot fungi, as well as several Trichoderma spp. which are known to offer a wide variety of degradative capabilities. To study the basic mechanisms of microbial degradation we plan to assess the relationships between the levels of ligninases (H2 and H8) and manganese peroxidase vs. degradative ability of the fungus on toxic chemicals. An effort will be made to study their metabolic systems to explain the fundamental mechanisms as well as toxicological significance of their metabolic alteration. Ultimately these efforts should result in maximization of degradation abilities of these organisms. At the same time, we plan to combine the above microbial technology with photochemical degradation technology. The P. chrysosporium method is particularly suited for this combined approach, since they are the most ultraviolet resistant species among all white-rot fungi we tested, and since they generated H2O2 which promotes photochemical degradation of organic chemicals. Furthermore, they grow well at liquid surfaces where oxygen tension is high and UV light can easily reach. Photochemical reactions will be first studied as a separate treatment and second combined with microbial actions. The basic cause(s) for synergism of these two technologies will be studied by determining their mechanisms and the nature of the products.

Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
Taha, Ameer Y; Hennebelle, Marie; Yang, Jun et al. (2018) Regulation of rat plasma and cerebral cortex oxylipin concentrations with increasing levels of dietary linoleic acid. Prostaglandins Leukot Essent Fatty Acids 138:71-80
Hill 3rd, Thomas; Rice, Robert H (2018) DUOX expression in human keratinocytes and bronchial epithelial cells: Influence of vanadate. Toxicol In Vitro 46:257-264
Kodani, Sean D; Wan, Debin; Wagner, Karen M et al. (2018) Design and Potency of Dual Soluble Epoxide Hydrolase/Fatty Acid Amide Hydrolase Inhibitors. ACS Omega 3:14076-14086
Ren, Qian; Ma, Min; Yang, Jun et al. (2018) Soluble epoxide hydrolase plays a key role in the pathogenesis of Parkinson's disease. Proc Natl Acad Sci U S A 115:E5815-E5823
Pecic, Stevan; Zeki, Amir A; Xu, Xiaoming et al. (2018) Novel piperidine-derived amide sEH inhibitors as mediators of lipid metabolism with improved stability. Prostaglandins Other Lipid Mediat 136:90-95
Yamanashi, Haruto; Boeglin, William E; Morisseau, Christophe et al. (2018) Catalytic activities of mammalian epoxide hydrolases with cis and trans fatty acid epoxides relevant to skin barrier function. J Lipid Res 59:684-695
Wang, Fuli; Zhang, Hongyong; Ma, Ai-Hong et al. (2018) COX-2/sEH Dual Inhibitor PTUPB Potentiates the Antitumor Efficacy of Cisplatin. Mol Cancer Ther 17:474-483
Napimoga, M H; Rocha, E P; Trindade-da-Silva, C A et al. (2018) Soluble epoxide hydrolase inhibitor promotes immunomodulation to inhibit bone resorption. J Periodontal Res 53:743-749
Blöcher, René; Wagner, Karen M; Gopireddy, Raghavender R et al. (2018) Orally Available Soluble Epoxide Hydrolase/Phosphodiesterase 4 Dual Inhibitor Treats Inflammatory Pain. J Med Chem 61:3541-3550
Hao, Lei; Kearns, Jamie; Scott, Sheyenne et al. (2018) Indomethacin Enhances Brown Fat Activity. J Pharmacol Exp Ther 365:467-475

Showing the most recent 10 out of 1149 publications