The Aquatic Toxicology Core Facility provides technical expertise and facilities for the rearing, holding and toxicological testing of aquatic organisms. The aquatic core facility was completed in 1999 through funding from the University of Florida's College of Veterinary Medicine and Institute for Food and Agricultural Sciences, and currently operates on a cost-recovery basis. The incentive for establishment of the aquatic facility was based upon the extensive research efforts in aquatic toxicology form a number of University of Florida researchers and the lack of other aquatic toxicology facilities available. Accordingly, financial support for this core is essential for the successful completion of those projects which involving holding and toxicant dosing of aquatic animals, including project #1 (Drs. Denslow and Gallagher), project #2 (Drs. Schoeb and Gross), and project #6 (Drs. James and Kleinow).
The specific aims of the core are 1) to provide Superfund investigators with technical assistance and consultation during all aquatic experiments involving the rearing, husbandry and dosing of aquatic organisms, and 2) to provide a physical environment for carrying out aquatic toxicological studies pertinent to the Superfund Program project grant. The aquatic core facility has the capability to house multiple aquatic species and to conduct acute and chronic studies. Project investigators will work with the aquatic core manager in the design and execution of all in vivo aquatic studies. A maximum amount of flexibility has been designed into the facility, allowing the facilities to be adapted to an investigator's needs. The facility is supplied with dechlorinated city water at a maximum rate of 20,000 gpm and three-1hp regenerative blowers at a maximum rate of 70 cfm supply aeration throughout the facility. All effluent is removed via a central drain system. Included as a service of the facility is an evaluation of the investigator's protocols by the ATD advisory board. This board approves projects to be conducted with in the facility and makes suggestions concerning animal husbandry and effluent discharge. The facility also has trained staff to perform water quality analysis and disease diagnosis and treatment.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
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Special Emphasis Panel (ZES1-MAO-A (G1))
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University of Florida
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Mangal, Naveen; James, Margaret O; Stacpoole, Peter W et al. (2018) Model Informed Dose Optimization of Dichloroacetate for the Treatment of Congenital Lactic Acidosis in Children. J Clin Pharmacol 58:212-220
Jiang, Yu; Milavetz, Gary; James, Margaret O et al. (2017) A Mechanism-Based Pharmacokinetic Enzyme Turnover Model for Dichloroacetic Acid Autoinhibition in Rats. J Pharm Sci 106:1396-1404
Shroads, Albert L; Coats, Bonnie S; Langaee, Taimour et al. (2015) Chloral hydrate, through biotransformation to dichloroacetate, inhibits maleylacetoacetate isomerase and tyrosine catabolism in humans. Drug Metab Pers Ther 30:49-55
Shroads, A L; Coats, B S; McDonough, C W et al. (2015) Haplotype variations in glutathione transferase zeta 1 influence the kinetics and dynamics of chronic dichloroacetate in children. J Clin Pharmacol 55:50-5
James, Margaret O; Kleinow, Kevin M (2014) Seasonal influences on PCB retention and biotransformation in fish. Environ Sci Pollut Res Int 21:6324-33
Garcia-Reyero, Natàlia; Martyniuk, Christopher J; Kroll, Kevin J et al. (2013) Transcriptional signature of progesterone in the fathead minnow ovary (Pimephales promelas). Gen Comp Endocrinol 192:159-69
Kocerha, Jannet; Prucha, Melinda S; Kroll, Kevin J et al. (2010) Regulation of steroidogenic acute regulatory protein transcription in largemouth bass by orphan nuclear receptor signaling pathways. Endocrinology 151:341-9
Tan, Xiaobing; Yim, Sun-Young; Uppu, Prasanna et al. (2010) Enhanced bioaccumulation of dietary contaminants in catfish with exposure to the waterborne surfactant linear alkylbenzene sulfonate. Aquat Toxicol 99:300-8
Nyagode, Beatrice A; James, Margaret O; Kleinow, Kevin M (2009) Influence of dietary Coexposure to benzo(a)pyrene on the biotransformation and distribution of 14C-methoxychlor in the channel catfish (Ictalurus punctatus). Toxicol Sci 108:320-9
Rauschenberger, R Heath; Sepulveda, Maria S; Wiebe, Jon J et al. (2009) Nutrient and organochlorine pesticide concentrations in American alligator eggs and their associations with clutch viability. J Aquat Anim Health 21:249-61

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