The Cell Sciences and Immunochemistry Core (CIC) is an integral part of the SLE SCOR Center, providing services to the four principal investigators on projects that rely on the following laboratory support. First, routine histology and electron microscopy studies and their interpretations. Second, tissue and cellular localization of antigenic molecules by immunohistology, and messenger RNA (mRNA) by in situ hybridization. Third, acquisition and management of human and murine recombinant proteins and peptide libraries for the """"""""lupus antigens"""""""" including Ro60, Ro52, La, Sm, calreticulin, U1-70 RNAP, dsDNA and phospholipids. Fourth acquisition and management of a library of essential monoclonal Ab and cDNA probes for Ag localization and flow cytometry, as well as B cell hybridomas generating sufficient monoclonal Ab for in vivo experiments. Fifth, quantitation and isotype determination of human and murine autoAb by immunoprecipitation and ELISA. Sixth, quantitation of murine cytokines by ELISA. The CIC will function, in collaboration with the project investigators, in experiments that: 1) analyze autoAb responses to the """"""""lupus Ag"""""""" and their overlapping peptides, 2) require peptides for mapping their T and B cell epitopes, 3) evaluate the immunopathologic end points including the detection and semi-quantitation glomerulonephritis, sialoadenitis and diseases affecting other organs that result from autoimmune response to the lupus Ag, 4) evaluate the quality of the autoimmune response with respect to the specificity and Ig isotype of the autoAb and the nature of the cytokines produces by peptide specific T cells. In summary, the CIC will support the research project of the SLE SCOR by providing common reagents and by conducting several essential and common techniques.

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Sung, Sun-Sang J; Ge, Yan; Dai, Chao et al. (2017) Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol 198:2589-2601
Dai, Chao; Deng, Yun; Quinlan, Aaron et al. (2014) Genetics of systemic lupus erythematosus: immune responses and end organ resistance to damage. Curr Opin Immunol 31:87-96
Ge, Yan; Jiang, Chao; Sung, Sun-Sang J et al. (2013) Cgnz1 allele confers kidney resistance to damage preventing progression of immune complex-mediated acute lupus glomerulonephritis. J Exp Med 210:2387-401
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Deshmukh, Umesh S; Sim, Davis L; Dai, Chao et al. (2011) HLA-DR3 restricted T cell epitope mimicry in induction of autoimmune response to lupus-associated antigen SmD. J Autoimmun 37:254-62
Jiang, Chao; Deshmukh, Umesh S; Gaskin, Felicia et al. (2010) Differential responses to Smith D autoantigen by mice with HLA-DR and HLA-DQ transgenes: dominant responses by HLA-DR3 transgenic mice with diversification of autoantibodies to small nuclear ribonucleoprotein, double-stranded DNA, and nuclear antigens. J Immunol 184:1085-91
Sharma, Rahul; Sung, Sun-sang Joe; Fu, Shu Man et al. (2009) Regulation of multi-organ inflammation in the regulatory T cell-deficient scurfy mice. J Biomed Sci 16:20
Bagavant, Harini; Fu, Shu Man (2009) Pathogenesis of kidney disease in systemic lupus erythematosus. Curr Opin Rheumatol 21:489-94

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