The Molecular Genetics Core Laboratory (MGCL) will provide an important resource to the TTURC based upon existing sample handling, biorepository and high throughput assay procedures. This core will be within Dr. Shields' Molecular Epidemiology Section the Laboratory of Human Carcinogenesis (LHC) within NCI. The MGCL is uniquely suited to provide the laboratory genetic support for existing hypotheses and to develop assays for new hypotheses.
Our specific aims are: (1) to provide a certified Molecular Genetics Core Laboratory (MGCL) that ensures consistent and high quality sample handling, analysis and reporting for samples generated by the TTURC; (2) to provide sample receipt, sample processing and biorepository procedures for all TTURC samples; (3) to provide existing consistent methods for DNA extraction and genetic polymorphism analysis; and (4) to provide accurate reporting, quality control and quality assurance procedures. The MGCL will maintain CLIA certification for quality control and quality assurance, among existing procedures for accurate determination of assay results and reporting. Performing large-scale molecular epidemiology studies require experience and expertise. Our laboratory has done DNA extractions. from blood (white blood cells, blood clots and serum), buccal swabs, oral rinses, frozen human organ tissue (lung, liver, colon. breast), urine and paraffin-embedded tissues. During the last five years, this constitutes DNA from more than 2,000 persons. Our success rates are greater than 98%. In calendar years 1996, 1997 and 1998, we performed 21 different genotyping assays for a total of 24,315 individual assays. There are different ways for laboratories to perform quality research. Ours is to maintain Clinical Laboratory Improvement Amendments (CLIA) certification. This is a government-mandated quality control and quality assurance monitoring program for clinical laboratories.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
1P50CA084718-01
Application #
6257859
Study Section
Special Emphasis Panel (ZCA1-SRRB-Y (O2))
Project Start
1999-09-30
Project End
2004-09-29
Budget Start
Budget End
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
Hsu, Ping-Ching; Lan, Renny S; Brasky, Theodore M et al. (2017) Metabolomic profiles of current cigarette smokers. Mol Carcinog 56:594-606
Hsu, Ping-Ching; Lan, Renny S; Brasky, Theodore M et al. (2017) Menthol Smokers: Metabolomic Profiling and Smoking Behavior. Cancer Epidemiol Biomarkers Prev 26:51-60
Weng, Daniel Y; Chen, Jinguo; Taslim, Cenny et al. (2016) Persistent alterations of gene expression profiling of human peripheral blood mononuclear cells from smokers. Mol Carcinog 55:1424-37
Song, Min-Ae; Marian, Catalin; Brasky, Theodore M et al. (2016) Chemical and toxicological characteristics of conventional and low-TSNA moist snuff tobacco products. Toxicol Lett 245:68-77
Schnoll, Robert A; Goelz, Patricia M; Veluz-Wilkins, Anna et al. (2015) Long-term nicotine replacement therapy: a randomized clinical trial. JAMA Intern Med 175:504-11
David, Sean P; Strong, David R; Leventhal, Adam M et al. (2013) Influence of a dopamine pathway additive genetic efficacy score on smoking cessation: results from two randomized clinical trials of bupropion. Addiction 108:2202-11
Bough, K J; Lerman, C; Rose, J E et al. (2013) Biomarkers for smoking cessation. Clin Pharmacol Ther 93:526-38
Perkins, Kenneth A; Parzynski, Craig; Mercincavage, Melissa et al. (2012) Is self-efficacy for smoking abstinence a cause of, or a reflection on, smoking behavior change? Exp Clin Psychopharmacol 20:56-62
Conklin, Cynthia A; Parzynski, Craig S; Salkeld, Ronald P et al. (2012) Cue reactivity as a predictor of successful abstinence initiation among adult smokers. Exp Clin Psychopharmacol 20:473-8
Featherstone, Robert E; Phillips, Jennifer M; Thieu, Tony et al. (2012) Nicotine receptor subtype-specific effects on auditory evoked oscillations and potentials. PLoS One 7:e39775

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