Because of the translational requirement of SPORE research, it is essential that SPORE investigators have access to and assistance with animal models for therapeutic hypothesis testing. The UCSF Brain Tumor SPORE Animal Core will address this need by utilizing serially-passaged human GBM xenografts in conducting in vivo studies using immune-deficient mice and rats. Emphasis will be placed on the use of serially-passaged xenografts since our cumulative experience with this system of tumor propagation indicates that serial in vivo passaging promotes tumor retention of key properties that are lost during cell line establishment. In additon, the Animal Core will emphasize orthotopic (intracranial) therapeutic testing because intracranial tumors established from serially propagated flank xenografts are highly invasive, and therefore present a more rigorous and clinically-relevant test of therapeutic efficacy than would be provided by the well-circumscribed subcutaneous tumors propagated in the flanks of nude mice, or by well-circumscribed intracranial tumors that are produced by injection of established cell lines. In association with this test model philosophy, the objectives of the UCSF Brain Tumor Animal Core are as follows:
Aim 1 : Propagate, analyze (histopathological and molecular), archive, and maintain up-to-date records on all GBM xenografts used in support of SPORE animal model research.
Aim 2 : Coordinate and conduct all immune deficient rodent therapeutics testing, including optical imaging and molecular analyses of intracranial xenograft specimens, in assessing tumor response to therapy.
Aim 3 : In association with the Tissue and Outcome Core, utilize xenograft resources to facilitate interpretation of results from immunohistochemical and FISH analysis of therapeutic targets and/or surrogate markers in patient tumors.
Aim 4 : Collect, process, and distribute, within and outside of UCSF, xenograft tumor tissues and tissue extracts, so as to promote intra- and inter-SPORE collaborations, and utilization of these renewable tumor resources.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
5P50CA097257-10
Application #
8258664
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
2013-04-30
Budget Start
2011-05-01
Budget End
2013-04-30
Support Year
10
Fiscal Year
2011
Total Cost
$115,531
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Ostrom, Quinn T; Kinnersley, Ben; Wrensch, Margaret R et al. (2018) Sex-specific glioma genome-wide association study identifies new risk locus at 3p21.31 in females, and finds sex-differences in risk at 8q24.21. Sci Rep 8:7352
Salas, Lucas A; Koestler, Devin C; Butler, Rondi A et al. (2018) An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArray. Genome Biol 19:64
Choi, Serah; Yu, Yao; Grimmer, Matthew R et al. (2018) Temozolomide-associated hypermutation in gliomas. Neuro Oncol 20:1300-1309
Jacobs, Daniel I; Liu, Yanhong; Gabrusiewicz, Konrad et al. (2018) Germline polymorphisms in myeloid-associated genes are not associated with survival in glioma patients. J Neurooncol 136:33-39
Berntsson, Shala G; Merrell, Ryan T; Amirian, E Susan et al. (2018) Glioma-related seizures in relation to histopathological subtypes: a report from the glioma international case-control study. J Neurol 265:1432-1442
Goode, Benjamin; Joseph, Nancy M; Stevers, Meredith et al. (2018) Adenomatoid tumors of the male and female genital tract are defined by TRAF7 mutations that drive aberrant NF-kB pathway activation. Mod Pathol 31:660-673
Hayes, Josie; Yu, Yao; Jalbert, Llewellyn E et al. (2018) Genomic analysis of the origins and evolution of multicentric diffuse lower-grade gliomas. Neuro Oncol 20:632-641
Ostrom, Quinn T; Kinnersley, Ben; Armstrong, Georgina et al. (2018) Age-specific genome-wide association study in glioblastoma identifies increased proportion of 'lower grade glioma'-like features associated with younger age. Int J Cancer 143:2359-2366
Pekmezci, Melike; Stevers, Meredith; Phillips, Joanna J et al. (2018) Multinodular and vacuolating neuronal tumor of the cerebrum is a clonal neoplasm defined by genetic alterations that activate the MAP kinase signaling pathway. Acta Neuropathol 135:485-488
Behr, Spencer C; Villanueva-Meyer, Javier E; Li, Yan et al. (2018) Targeting iron metabolism in high-grade glioma with 68Ga-citrate PET/MR. JCI Insight 3:

Showing the most recent 10 out of 362 publications