The purpose of the organ culture and tissue processing core facility (core B) is to provide a centralized source of metanephroi for biochemical, tissue culture, in situ hybridization, and immunohistochemical analysis by the five participating laboratories. Obtaining and maintaining metanephroi entail considerable skill and experience already possessed by the personnel of the facility. This core will free the time and resources that the project directors would have had to otherwise devote to tissue preparation and facilitate their progression along the proposed lines of investigation. The facility will assure uniformity, allowing cross comparisons of the timing of observed changes in transport proteins, growth related genes and factors, and structure. The core will minimize duplication of equipment and decrease expenditures. The centralization of maintaining metanephric organ culture and tissue processing will assure uniformity and reproducibility of observations made in separate laboratories.

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Washington University
Saint Louis
United States
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Hammerman, Marc R (2004) Applications of organ precursor cell therapy: can lessons from embryonic kidney transplantation be applied to the endocrine pancreas? Curr Opin Nephrol Hypertens 13:23-9
Hammerman, Marc R (2004) Transplantation of embryonic organs - kidney and pancreas. Am J Transplant 4 Suppl 6:14-24
Akimoto, Tetsu; Hammerman, Marc R (2003) Fibroblast growth factor 2 promotes microvessel formation from mouse embryonic aorta. Am J Physiol Cell Physiol 284:C371-7
Rogers, Sharon A; Talcott, Michael; Hammerman, Marc R (2003) Transplantation of pig metanephroi. ASAIO J 49:48-52
Cheng, Hui-Teng; Miner, Jeffrey H; Lin, MeeiHua et al. (2003) Gamma-secretase activity is dispensable for mesenchyme-to-epithelium transition but required for podocyte and proximal tubule formation in developing mouse kidney. Development 130:5031-42
Rogers, Sharon A; Liapis, Helen; Hammerman, Marc R (2003) Intraperitoneal transplantation of pancreatic anlagen. ASAIO J 49:527-32
Holliday, L S; Welgus, H G; Hanna, J et al. (2003) Interstitial collagenase activity stimulates the formation of actin rings and ruffled membranes in mouse marrow osteoclasts. Calcif Tissue Int 72:206-14
Hammerman, Marc R (2003) Therapeutic promise of embryonic kidney transplantation. Nephron Exp Nephrol 93:e58
Hammerman, Marc R (2003) Applications of cell therapy to whole kidney replacement. Curr Opin Nephrol Hypertens 12:1-3
Kikkawa, Yamato; Virtanen, Ismo; Miner, Jeffrey H (2003) Mesangial cells organize the glomerular capillaries by adhering to the G domain of laminin alpha5 in the glomerular basement membrane. J Cell Biol 161:187-96

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