PROJECT 1 - IN (STROUD. PROJECT LEADER)Our goal is to understand the mechanism of HIV-1 integration mediated by integrase (IN), in thecontext of pre-integration complexes (PICs) that provide the targets for anti-IN drugs. The components ofPICs, and interactions with viral and cellular proteins will be defined. Our plan builds on our 1.6 A resolutionstructure of the isolated core domain (IN52'210), our 2.6 A structure of the two-domain catalytic core plus Cterminaldomain (IN52'288), and our exclusive ability to prepare homogeneous thiol-linked IN-DNA complexes.We will further analyze the crystal structure of IN without and with its natural substrate, the viral att site andtarget DNA, and use that knowledge to determine the mechanisms of anti-IN therapeutics.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center (P50)
Project #
1P50GM082250-01
Application #
7480051
Study Section
Special Emphasis Panel (ZRG1-AARR-A (40))
Project Start
2007-09-01
Project End
2012-07-31
Budget Start
2007-09-01
Budget End
2008-07-31
Support Year
1
Fiscal Year
2007
Total Cost
$421,694
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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