Core - Cell Culture

Welsh, Michael John

Abstract

The Cell Culture Core is established as a resource for all projects in the center. The Core provides several services to the individual projects: 1) It provides primary cell cultures, including CF epithelial cells, to participating investigators. 2) It provides cell lines for studies of the expression of recombinant CFTR. 3) It develops new culture conditions and substrates that allow the most efficient use of cells and provide the optimal conditions for individual investigators. 40 It provides investigators with recombinant vaccinia virus stocks. 5) It maintains hybridomas producing monoclonal antibodies and provides the antibodies to individual investigators.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Specialized Center (P50)
• Project #: 5P50HL042385-09
• Application #: 5213778
• Support Year: 9
• Fiscal Year: 1996

Publications

Graham, Scott M; Scott, Shaun N; Launspach, Janice et al. (2002) The effects of fluticasone propionate on nasal epithelial potential difference. Am J Rhinol 16:145-9

Welsh, Michael J; Price, Margaret P; Xie, Jinghui (2002) Biochemical basis of touch perception: mechanosensory function of degenerin/epithelial Na+ channels. J Biol Chem 277:2369-72

Ostedgaard, L S; Baldursson, O; Welsh, M J (2001) Regulation of the cystic fibrosis transmembrane conductance regulator Cl- channel by its R domain. J Biol Chem 276:7689-92

Sawai, M V; Jia, H P; Liu, L et al. (2001) The NMR structure of human beta-defensin-2 reveals a novel alpha-helical segment. Biochemistry 40:3810-6

Prince, L S; Karp, P H; Moninger, T O et al. (2001) KGF alters gene expression in human airway epithelia: potential regulation of the inflammatory response. Physiol Genomics 6:81-9

Zabner, J; Seiler, M P; Launspach, J L et al. (2000) The osmolyte xylitol reduces the salt concentration of airway surface liquid and may enhance bacterial killing. Proc Natl Acad Sci U S A 97:11614-9

Jepsen, M; Graham, S; Karp, P H et al. (2000) Effect of topical nasal pharmaceuticals on sodium and chloride transport by human airway epithelia. Am J Rhinol 14:405-9

Ostedgaard, L S; Zeiher, B; Welsh, M J (1999) Processing of CFTR bearing the P574H mutation differs from wild-type and deltaF508-CFTR. J Cell Sci 112 ( Pt 13):2091-8

Cotten, J F; Welsh, M J (1999) Cystic fibrosis-associated mutations at arginine 347 alter the pore architecture of CFTR. Evidence for disruption of a salt bridge. J Biol Chem 274:5429-35

McCray Jr, P B; Zabner, J; Jia, H P et al. (1999) Efficient killing of inhaled bacteria in DeltaF508 mice: role of airway surface liquid composition. Am J Physiol 277:L183-90