Core - Blood Products

Abstract

This laboratory core facility will be responsible for obtaining and providing samples of blood, bone marrow, and mobilized peripheral blood mononuclear cells to the investigators involved with this grant application. In addition, this laboratory will be specifically responsible for the collection and initial enrichment of CD34+ cells in support of the proposed clinical trial. The Clinical Cryobiology Laboratory is an essential resource of the Hutchinson Center providing marrow and peripheral blood stem cell harvesting, in vitro processing (including enrichment and depletion of specific cell populations), cryopreservation, and storage services for healthy donors and patients.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Specialized Center (P50)
• Project #: 5P50HL054881-02
• Application #: 2374624
• Budget Start: 1995-10-01
• Budget End: 1996-09-30
• Support Year: 2
• Fiscal Year: 1996

Institution

• Name: Fred Hutchinson Cancer Research Center
• DUNS #: 075524595
• City: Seattle
• State: WA
• Country: United States
• Zip Code: 98109

Publications

Varnum-Finney, Barbara; Dallas, Mari H; Kato, Keizo et al. (2008) Notch target Hes5 ensures appropriate Notch induced T- versus B-cell choices in the thymus. Blood 111:2615-20

Piasecki, Julia C; Beagles, Karen; Beard, Brian C et al. (2008) Induction of transgene-specific cytotoxic T lymphocyte responses after transplantation of gene-modified CD34+ cells despite nonablative immunosuppressive conditioning. Hum Gene Ther 19:103-7

Dallas, Mari H; Varnum-Finney, Barbara; Martin, Paul J et al. (2007) Enhanced T-cell reconstitution by hematopoietic progenitors expanded ex vivo using the Notch ligand Delta1. Blood 109:3579-87

Shepherd, Bryan E; Kiem, Hans-Peter; Lansdorp, Peter M et al. (2007) Hematopoietic stem-cell behavior in nonhuman primates. Blood 110:1806-13

Gerull, Sabine; Beard, Brian C; Peterson, Laura J et al. (2007) In vivo selection and chemoprotection after drug resistance gene therapy in a nonmyeloablative allogeneic transplantation setting in dogs. Hum Gene Ther 18:451-6

Jung, Chul Won; Beard, Brian C; Morris, Julia C et al. (2007) Hematopoietic stem cell engraftment: a direct comparison between intramarrow and intravenous injection in nonhuman primates. Exp Hematol 35:1132-9

Si, Jutong; Mueller, LeMoyne; Collins, Steven J (2007) CaMKII regulates retinoic acid receptor transcriptional activity and the differentiation of myeloid leukemia cells. J Clin Invest 117:1412-21

Neff, Tobias; Gerull, Sabine; Peterson, Laura J et al. (2007) Improved short-term engraftment of lentivirally versus gammaretrovirally transduced allogeneic canine repopulating cells. J Gene Med 9:357-61

Si, Jutong; Mueller, LeMoyne; Schuler, Aaron et al. (2007) The retinoic acid receptor/CaMKII interaction: pharmacologic inhibition of CaMKII enhances the differentiation of myeloid leukemia cells. Blood Cells Mol Dis 39:307-15

Aoyama, Keisuke; Delaney, Colleen; Varnum-Finney, Barbara et al. (2007) The interaction of the Wnt and Notch pathways modulates natural killer versus T cell differentiation. Stem Cells 25:2488-97