Abstract

The principal investigator, Cassandra Smith (along with the co-principal investigator, Charles Cantor) pioneered some of the physical methods that are used in conventional positional cloning of disease genes. These physical methods will be applied to Projects I (the human gene search) and Project II (the mouse gene search) once genetic studies narrow the search region. It is quite clear that conventional positional cloning methods will work for essential any disease gene provided that the appropriate samples and resources are available. However, it is also clear that the conventional methods are so inefficient (and expensive) that they cannot be used to uncover all human disease genes. Hence, this group has focused on developing and applying a number of novel physical methods and approaches that should considerably increase the efficiency of gene searches. Specifically, these methods focus on parallel processing and coincident detection schemes that allow the analysis of large numbers of samples en masse such as genetic markers and cDNAs, and the generation of useful candidate sequences from selected genomic regions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL055001-05
Application #
6302396
Study Section
Project Start
2000-02-01
Project End
2001-01-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2000
Total Cost
$327,999
Indirect Cost

  Institution

Name
Boston University
Department
Type
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Duka, Arvi; Schwartz, Faina; Duka, Irena et al. (2006) A novel gene (Cmya3) induced in the heart by angiotensin II-dependent but not salt-dependent hypertension in mice. Am J Hypertens 19:275-81
Shenouda, Sherene M; Johns, Conrado; Kintsurashvili, Ekaterina et al. (2006) Long-term inhibition of the central alpha(2B)-adrenergic receptor gene via recombinant AAV-delivered antisense in hypertensive rats. Am J Hypertens 19:1135-43
Cardoso de Carvalho, Fabio; Bregagnollo, Edson; Santos Silva, Vanessa et al. (2006) Frequency of coronary artery disease in patients with renal artery stenosis without clinical manifestations of coronary insufficiency. Am J Hypertens 19:1125-8
Cui, Jing; Melista, Efthymia; Chazaro, Irmarie et al. (2005) Sequence variation of bradykinin receptors B1 and B2 and association with hypertension. J Hypertens 23:55-62
Manolis, Athanasios J; Iraklianou, Stella; Pittaras, Andreas et al. (2005) Arterial compliance changes in diabetic normotensive patients after angiotensin-converting enzyme inhibition therapy. Am J Hypertens 18:18-22
Ignjacev-Lazich, Ivana; Kintsurashvili, Ekaterina; Johns, Conrado et al. (2005) Angiotensin-converting enzyme regulates bradykinin receptor gene expression. Am J Physiol Heart Circ Physiol 289:H1814-20
Russo, Christopher J; Melista, Efthymia; Cui, Jing et al. (2005) Association of NEDD4L ubiquitin ligase with essential hypertension. Hypertension 46:488-91
Schwartz, Faina; Duka, Arvi; Duka, Irena et al. (2004) Novel targets of ANG II regulation in mouse heart identified by serial analysis of gene expression. Am J Physiol Heart Circ Physiol 287:H1957-66
Elijovich, Fernando; Laffer, Cheryl L; Schiffrin, Ernesto L et al. (2004) Endothelin-aldosterone interaction and proteinuria in low-renin hypertension. J Hypertens 22:573-82
Erlich, Porat M; Cui, Jing; Chazaro, Irmarie et al. (2003) Genetic variants of WNK4 in whites and African Americans with hypertension. Hypertension 41:1191-5

Showing the most recent 10 out of 37 publications