Abstract

The Virology Core will provide fundamental, well-established services in respiratory virology to project investigators for Projects 1, 2, 3, 4 and 5. Dr. Frederick Henderson will be the Virology Core director and Dr. Melinda A. Beck will be the Co-Director. The virology core will provide the following services: 1) Isolation of viruses in respiratory secretions of children with acquired acute respiratory illnesses or adults with experimental infections, 2) Identification of viruses from nasal secretions (antigen detection by ELISA and FA detection of antigen), 3) Quantitation of virus (RSV, influenza) from respiratory secretions or organs from experimentally infected animals, 4) Preparation of virus stocks, including providing gradient purified viruses, 5) measuring antiviral antibody, both binding and neutralizing, 6) Development of monoclonal antibodies requested by the Projects. Dr. Henderson and Beck will provide hands-on laboratory work as well as providing technical assistance to the technician, Ms. Huang. Dr. HU will assist with the development of monoclonal antibody production.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Specialized Center (P50)
Project #
5P50HL056395-03
Application #
6110685
Study Section
Project Start
1998-12-01
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Mellow, Thomas E; Murphy, Paula C; Carson, Johnny L et al. (2004) The effect of respiratory synctial virus on chemokine release by differentiated airway epithelium. Exp Lung Res 30:43-57
Thai, C H; Gambling, T M; Carson, J L (2002) Freeze fracture study of airway epithelium from patients with primary ciliary dyskinesia. Thorax 57:363-5
Veness-Meehan, Kathleen A; Pierce, Richard A; Moats-Staats, Billie M et al. (2002) Retinoic acid attenuates O2-induced inhibition of lung septation. Am J Physiol Lung Cell Mol Physiol 283:L971-80
Carson, Johnny L; Reed, William; Lucier, Thomas et al. (2002) Axonemal dynein expression in human fetal tracheal epithelium. Am J Physiol Lung Cell Mol Physiol 282:L421-30
Kazachkov, Mikhail Y; Hu, P C; Carson, Johnny L et al. (2002) Release of cytokines by human nasal epithelial cells and peripheral blood mononuclear cells infected with Mycoplasma pneumoniae. Exp Biol Med (Maywood) 227:330-5
Noah, Terry L; Ivins, Sally S; Murphy, Paula et al. (2002) Chemokines and inflammation in the nasal passages of infants with respiratory syncytial virus bronchiolitis. Clin Immunol 104:86-95
Price, W A; Lee, E; Maynor, A et al. (2001) Relation between serum insulinlike growth factor-1, insulinlike growth factor binding protein-2, and insulinlike growth factor binding protein-3 and nutritional intake in premature infants with bronchopulmonary dysplasia. J Pediatr Gastroenterol Nutr 32:542-9
Volberg, T; Romer, L; Zamir, E et al. (2001) pp60(c-src) and related tyrosine kinases: a role in the assembly and reorganization of matrix adhesions. J Cell Sci 114:2279-89
Veness-Meehan, K A; Bottone Jr, F G; Stiles, A D (2000) Effects of retinoic acid on airspace development and lung collagen in hyperoxia-exposed newborn rats. Pediatr Res 48:434-44
Reed, W; Carson, J L; Moats-Staats, B M et al. (2000) Characterization of an axonemal dynein heavy chain expressed early in airway epithelial ciliogenesis. Am J Respir Cell Mol Biol 23:734-41

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