One of the most difficult components of clinical and translational research is the task of monitoring diseaseactivity and developing insights into the mechanisms of disease. One well established method of addressingthese issues is the measurement of biomarkers from human and animal specimens. The goal of this Core isto provide for the measurement of biomarkers in samples derived from the individual projects of this SCCORprogram. In order to accomplish this goal, we will offer these individual projects a uniform resource for thelarge-scale measurement of biomarkers in plasma, serum, and urine. We will also use tissue culturemethods to develop reporter assays that will employ the exposure of patient samples to cultured cells andmeasuring the evoked response. We will also centralize and organize the storage of samples from theindividual projects so that samples are readily available for assay with any new or emerging biomarkers thatbecome available during the course of this project. The principal means of measuring biomarkers in thiscore unit will be commercially available enzyme-linked immunoabsorbant assays (ELISAs) orradioimmunoassays (RIAs). Because some assays may not be commercially available, this core will alsogenerate in house ELISA and RIA methods using commercially-available antibodies. This core unit willgenerate data that spans all projects of the SCCOR program and thus, should provide a common basis forcomparison between animal models and human disease.
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