The goal of the Bio-Analytical Redox Biology (BARB) core is to provide state-of-the-art services in mitochondrial damage, function, and proteomics; oxidative stress assessment; and quantification of atherosclerosis. These services address key processes contributing to both metabolic and vascular pathology in diabetes, diabetes complications, and cardiometabolic disease. Core technologies are based on the expressed needs of funded DRTC investigators, The specific aims of the BARB core will be: 1) Provide services to DRTC Regular and Supported Members and pilot & feasibility grant awardees in: (i) mitochondrial bioanalytical analysis (multiple mitochondrial functions, mtDNA damage and haplotyping), (ii) mitochondrial proteomics (quantitative proteomics, post-translational oxidative modifications of proteins), (iii) oxidative stress assessment (isoprostanes, oxidized lipids, aconitase inactivation, GSH/GSSG, myeloperoxidase, heme oxygenase, and antioxidants), and (iv) atherosclerotic lesion quantification using state-of-the-art research facilities and specialized expertise, with emphasis on quality control of study procedures. 2) To substantially reduce cost and investigator time commitment by accessing centralized resources, as opposed to operating independent laboratories and hiring separate faculty/staff. 3) To provide investigators and their technical personnel opportunities to learn and to further develop new research methodologies through recurring workshops and initial consultations to enable optimal usage of the various services of this core. 4) Pursue and develop methodologies that allow the investigator to extend capabilities and precision in redox and free radical biology, and to foster multi-disciplinary collaborations. Because analytical technologies are continually evolving, an important role of the BARB core will be to keep DRTC investigators appraised of current technological developments in the field through a program of tutorials, seminars and mitochondrial interest group (MIG) meetings (already initiated); this will be especially important for DRTC investigators who need to employ or extend the use of these technologies in their hypothesis testing

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Comprehensive Center (P60)
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Special Emphasis Panel (ZDK1-GRB-S (O1))
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University of Alabama Birmingham
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Kang, Minsung; Liu, Xiaobing; Fu, Yuchang et al. (2018) Improved systemic metabolism and adipocyte biology in miR-150 knockout mice. Metabolism 83:139-148
Jo, SeongHo; Chen, Junqin; Xu, Guanlan et al. (2018) miR-204 Controls Glucagon-Like Peptide 1 Receptor Expression and Agonist Function. Diabetes 67:256-264
Mohler 3rd, Emile R; Ellenberg, Susan S; Lewis, Cora E et al. (2018) The Effect of Testosterone on Cardiovascular Biomarkers in the Testosterone Trials. J Clin Endocrinol Metab 103:681-688
Hunter, Gary R; Bryan, David R; Borges, Juliano H et al. (2018) Racial Differences in Relative Skeletal Muscle Mass Loss During Diet-Induced Weight Loss in Women. Obesity (Silver Spring) 26:1255-1260
Wingo, Brooks C; Barry, Valene Garr; Ellis, Amy C et al. (2018) Comparison of segmental body composition estimated by bioelectrical impedance analysis and dual-energy X-ray absorptiometry. Clin Nutr ESPEN 28:141-147
Hunter, Gary R; Plaisance, Eric P; Carter, Stephen J et al. (2018) Why intensity is not a bad word: Optimizing health status at any age. Clin Nutr 37:56-60
Engle, Staci E; Antonellis, Patrick J; Whitehouse, Logan S et al. (2018) A CreER mouse to study melanin concentrating hormone signaling in the developing brain. Genesis 56:e23217
Hunter, Gary R; Fisher, Gordon; Bryan, David R et al. (2018) Divergent Blood Pressure Response After High-Intensity Interval Exercise: A Signal of Delayed Recovery? J Strength Cond Res 32:3004-3010
Snyder, Peter J; Bhasin, Shalender; Cunningham, Glenn R et al. (2018) Lessons From the Testosterone Trials. Endocr Rev 39:369-386
Ingram, K H; Hunter, G R; James, J F et al. (2017) Central fat accretion and insulin sensitivity: differential relationships in parous and nulliparous women. Int J Obes (Lond) 41:1214-1217

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