The respiratory burst oxidase (RBO) is the enzyme responsible for superoxide production by phagocytes and B lymphocytes. It is dormant in resting cells but comes to life when the cells are activated. Among the components of the RBO is a 47K (formerly 48K) protein known as p47phox that becomes phosphorylated when RBO-containing cells are activated.
The aims of the present work are to identify the sites of phosphorylation in p47phox and elucidate their relationship to the activity of the RBO; to identify proteins with which p47phox interacts and understand these interactions at a molecular level; and to produce large quantities of p47phox for structure determination by X- ray crystallography.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
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Hematology Subcommittee 2 (HEM)
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Scripps Research Institute
La Jolla
United States
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