Abstract

The respiratory burst oxidase (RBO) is the enzyme responsible for superoxide production by phagocytes and B lymphocytes. It is dormant in resting cells but comes to life when the cells are activated. Among the components of the RBO is a 47K (formerly 48K) protein known as p47phox that becomes phosphorylated when RBO-containing cells are activated.
The aims of the present work are to identify the sites of phosphorylation in p47phox and elucidate their relationship to the activity of the RBO; to identify proteins with which p47phox interacts and understand these interactions at a molecular level; and to produce large quantities of p47phox for structure determination by X- ray crystallography.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI028479-09
Application #
2671964
Study Section
Hematology Subcommittee 2 (HEM)
Project Start
1989-07-01
Project End
2000-03-31
Budget Start
1998-04-01
Budget End
1999-03-31
Support Year
9
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

DeLano, Frank A; Parks, Dale A; Ruedi, Julie M et al. (2006) Microvascular display of xanthine oxidase and NADPH oxidase in the spontaneously hypertensive rat. Microcirculation 13:551-66
Lopes, Lucia Rossetti; Dagher, Marie-Claire; Gutierrez, Abel et al. (2004) Phosphorylated p40PHOX as a negative regulator of NADPH oxidase. Biochemistry 43:3723-30
Babior, Bernard M (2004) NADPH oxidase. Curr Opin Immunol 16:42-7
Hoyal, Carolyn R; Gutierrez, Abel; Young, Brandon M et al. (2003) Modulation of p47PHOX activity by site-specific phosphorylation: Akt-dependent activation of the NADPH oxidase. Proc Natl Acad Sci U S A 100:5130-5
Catz, S D; Johnson, J L (2003) BCL-2 in prostate cancer: a minireview. Apoptosis 8:29-37
Dang, Pham My-Chan; Cross, Andrew R; Quinn, Mark T et al. (2002) Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II. Proc Natl Acad Sci U S A 99:4262-5
Catz, Sergio D; Johnson, Jennifer L; Babior, Bernard M (2002) The C2A domain of JFC1 binds to 3'-phosphorylated phosphoinositides and directs plasma membrane association in living cells. Proc Natl Acad Sci U S A 99:11652-7
Catz, Sergio D; Babior, Bernard M; Johnson, Jennifer L (2002) JFC1 is transcriptionally activated by nuclear factor-kappaB and up-regulated by tumour necrosis factor alpha in prostate carcinoma cells. Biochem J 367:791-9
Dang, P M; Cross, A R; Babior, B M (2001) Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558. Proc Natl Acad Sci U S A 98:3001-5
Babior, B M (2000) Phagocytes and oxidative stress. Am J Med 109:33-44

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