Enteric bacteria have related house keeping genes in about the same chromosomal order. The DNA differences between say Salmonella and E. coli appear to be sequences which are clustered together within islands of non-homologous DNA. These islands appear to contain most of the genes that are involved in pathogenesis and have been called pathogenicity islands although their origin, means of transmission, and precisely what they contain remain a mystery. There are two major aims in this proposal. One is to identify the loops or islands that distinguish various Salmonella (with different host preferences) from each other, from E. coli, and from other enteric bacteria. Also part of this aim is to construct Salmonella derivatives missing the islands or containing mutations in the islands and then characterize the phenotype of the derivative strains by a variety of virulence assays. The second major aim is to determine the role of genetic rearrangement in S. Typhi, if any. S. typhi and some S. Paratyphi can cause typhiod fever. The authors observed that these human adapted strains undergo genetic rearrangement at high frequency. In culture the same kinds of rearrangements have never been observed. The authors believe that the rearrangements are associated with growth in cells and will investigate this hypothesis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI034829-11
Application #
6169891
Study Section
Special Emphasis Panel (ZRG2-BIOL-1 (01))
Project Start
1989-12-15
Project End
2003-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
11
Fiscal Year
2000
Total Cost
$242,355
Indirect Cost
Name
Sidney Kimmel Cancer Center
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92121
Achtman, Mark; Hale, James; Murphy, Ronan A et al. (2013) Population structures in the SARA and SARB reference collections of Salmonella enterica according to MLST, MLEE and microarray hybridization. Infect Genet Evol 16:314-25
Evans, Matthew R; Fink, Ryan C; Vazquez-Torres, Andres et al. (2011) Analysis of the ArcA regulon in anaerobically grown Salmonella enterica sv. Typhimurium. BMC Microbiol 11:58
Arrach, Nabil; Cheng, Pui; Zhao, Ming et al. (2010) High-throughput screening for salmonella avirulent mutants that retain targeting of solid tumors. Cancer Res 70:2165-70
Frye, Jonathan G; Lindsey, Rebecca L; Rondeau, Gaelle et al. (2010) Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database. Microb Drug Resist 16:9-19
Thijs, Inge M; Zhao, Hui; De Weerdt, Ami et al. (2010) The AI-2-dependent regulator LsrR has a limited regulon in Salmonella Typhimurium. Cell Res 20:966-9
Xia, Xiao-Qin; McClelland, Michael; Porwollik, Steffen et al. (2009) WebArrayDB: cross-platform microarray data analysis and public data repository. Bioinformatics 25:2425-9
Andrews-Polymenis, Helene L; Santiviago, Carlos A; McClelland, Michael (2009) Novel genetic tools for studying food-borne Salmonella. Curr Opin Biotechnol 20:149-57
Merighi, Massimo; Septer, Alecia N; Carroll-Portillo, Amanda et al. (2009) Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar Typhimurium. BMC Microbiol 9:42
Santiviago, Carlos A; Reynolds, M Megan; Porwollik, Steffen et al. (2009) Analysis of pools of targeted Salmonella deletion mutants identifies novel genes affecting fitness during competitive infection in mice. PLoS Pathog 5:e1000477
Singh, Varsha; Mishra, Shrutkirti; Rao, G R K et al. (2008) Evaluation of nested PCR in detection of Helicobacter pylori targeting a highly conserved gene: HSP60. Helicobacter 13:30-4

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