This application proposes the functional characterization of mycobacterial sigma factors which the PI believes will be vital in facilitating regulated, rapid responses to the changing environments experienced by the bacilli during the course of their life cycle. The application is a continuation of an R29 award during which the PI identified sigma F (sigF) as an important regulator of loci implicated in stress and infection maintenance. Scanning of the MycD database reveals 13 putative sigma factors of which 10 are of the extracytoplasmic function group which in other systems have been shown to regulate cell wall synthesis pathways and protein secretion. The PI proposes to extend his studies by knocking out all of these sigma factors in both MTB and MAC. The knockouts will be characterized for both in vitro and in vivo phenotypes and the identity of sigma factor-dependent genes will be determined by microarray studies conducted on the knockout strains.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI036973-07
Application #
6169974
Study Section
Special Emphasis Panel (ZRG1-AARR-4 (01))
Program Officer
Sizemore, Christine F
Project Start
1994-09-30
Project End
2004-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
7
Fiscal Year
2000
Total Cost
$275,569
Indirect Cost
Name
Johns Hopkins University
Department
Public Health & Prev Medicine
Type
Schools of Public Health
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
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