The goal of the proposal is to develop an outer membrane protein F vaccine for the prevention of pulmonary infection with Pseudomonas aeruginosa. Linear B-cell epitopes of protein F may be protective against pseudomonas infection. In order to bypass immunogenicity problems of protein F or synthetic peptides, this proposal will use chimeric influenza viruses that contain OM protein F epitopes. Previous studies showed moderate efficacy in reduction of severity of pseudomonas pneumonia using a pilot vaccine, HG10-11. The current proposal will analyze the sera produced by these vaccines for antigen specificity and will further investigate the ability of the vaccine to modulate acute and chronic pseudomonas pneumonia. New vaccines with different sized OM peptides (9mer) will also be made and tested alone or in combination with 10-11 mer vaccine. Finally, chimeric tobacco mosaic virus vaccines will be constructed to evaluate their immunogenicity, functionality, and protective efficacy so as to utilize another viral vector for potential pseudomonas OM vaccines.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI044424-02
Application #
2887916
Study Section
Bacteriology and Mycology Subcommittee 2 (BM)
Program Officer
Taylor, Christopher E,
Project Start
1998-07-01
Project End
2001-06-30
Budget Start
1999-07-01
Budget End
2000-06-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Louisiana State University Hsc Shreveport
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
City
Shreveport
State
LA
Country
United States
Zip Code
71103
Staczek, J; Gilleland, L B; van der Heyde, H C et al. (2003) DNA vaccines against chronic lung infections by Pseudomonas aeruginosa. FEMS Immunol Med Microbiol 37:147-53
Price, Brian M; Barten Legutki, J; Galloway, Darrell R et al. (2002) Enhancement of the protective efficacy of an oprF DNA vaccine against Pseudomonas aeruginosa. FEMS Immunol Med Microbiol 33:89-99
Price, B M; Galloway, D R; Baker, N R et al. (2001) Protection against Pseudomonas aeruginosa chronic lung infection in mice by genetic immunization against outer membrane protein F (OprF) of P. aeruginosa. Infect Immun 69:3510-5
Staczek, J; Bendahmane, M; Gilleland, L B et al. (2000) Immunization with a chimeric tobacco mosaic virus containing an epitope of outer membrane protein F of Pseudomonas aeruginosa provides protection against challenge with P. aeruginosa. Vaccine 18:2266-74
Gilleland, H E; Gilleland, L B; Staczek, J et al. (2000) Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection. FEMS Immunol Med Microbiol 27:291-7