Abstract

We will continue our studies aimed at construction of mammalian cells with nonsense suppressors. We will construct, by in vitro methods, mutations in the anticodon region of cloned serine-tRNA genes from human DNA. These in vitro-made suppressor genes will be co-transformed into mammalian cells and tested for function with our previously characterized set of nonsense mutants in the TK gene of herpes simplex virus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA013515-13
Application #
3163779
Study Section
Virology Study Section (VR)
Project Start
1976-06-30
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code

  Publications

Xu, Yin; Stamenkovic, Ivan; Yu, Qin (2010) CD44 attenuates activation of the hippo signaling pathway and is a prime therapeutic target for glioblastoma. Cancer Res 70:2455-64
Glazer, P M; Sarkar, S N; Chisholm, G E et al. (1987) DNA mismatch repair detected in human cell extracts. Mol Cell Biol 7:218-24
Glazer, P M; Sarkar, S N; Summers, W C (1986) Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector. Proc Natl Acad Sci U S A 83:1041-4
Chisholm, G E; Summers, W C (1986) The promoter for the late gene encoding Vp5 of herpes simplex virus type 1 is recognized by cell extracts derived from uninfected cells. J Virol 60:620-5