Abstract

Our objectives are to identify genes or nucleotide sequences of nondefective murine C-type viruses that determine the ability of the viruses to induce leukemias in mice. We also wish to determine the genetic basis for the differences in the type of leukemia induced by different murine retroviruses. Our approach will be to sue genetics and biochemistry. In particular, we will construct site-specific rocombinants between molecularly cloned retroviruses to localize segments of the genome that confer the disease-inducing phenotype. The segments will be further dissected using the same approach, and ultimately, we will determine the nucleotide sequences of the critical regions and then attempt to subject them them to site-specific mutagenesis. These studies shoud provide insight into the role of non-defective C-type viral genomes in bringing about oncogenic transformation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA019308-12
Application #
3165131
Study Section
Virology Study Section (VR)
Project Start
1976-06-30
Project End
1988-11-30
Budget Start
1987-07-01
Budget End
1988-11-30
Support Year
12
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Kawakami, K; Hopkins, N (1996) Rapid identification of transgenic zebrafish. Trends Genet 12:9-10
Gaiano, N; Allende, M; Amsterdam, A et al. (1996) Highly efficient germ-line transmission of proviral insertions in zebrafish. Proc Natl Acad Sci U S A 93:7777-82
Amsterdam, A; Lin, S; Moss, L G et al. (1996) Requirements for green fluorescent protein detection in transgenic zebrafish embryos. Gene 173:99-103
Amsterdam, A; Lin, S; Hopkins, N (1995) The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos. Dev Biol 171:123-9
Manley, N R; O'Connell, M; Sun, W et al. (1993) Two factors that bind to highly conserved sequences in mammalian type C retroviral enhancers. J Virol 67:1967-75
Winandy, S; Renjifo, B; Li, Y et al. (1992) Nuclear factors that bind two regions important to transcriptional activity of the simian immunodeficiency virus long terminal repeat. J Virol 66:5216-23
Hunter, K; Housman, D; Hopkins, N (1991) Isolation and characterization of irradiation fusion hybrids from mouse chromosome 1 for mapping Rmc-1, a gene encoding a cellular receptor for MCF class murine retroviruses. Somat Cell Mol Genet 17:169-83
Renjifo, B; Speck, N A; Winandy, S et al. (1990) cis-acting elements in the U3 region of a simian immunodeficiency virus. J Virol 64:3130-4
Speck, N A; Renjifo, B; Golemis, E et al. (1990) Mutation of the core or adjacent LVb elements of the Moloney murine leukemia virus enhancer alters disease specificity. Genes Dev 4:233-42
Speck, N A; Renjifo, B; Hopkins, N (1990) Point mutations in the Moloney murine leukemia virus enhancer identify a lymphoid-specific viral core motif and 1,3-phorbol myristate acetate-inducible element. J Virol 64:543-50

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