The limited successes in nonhuman primate studies in eliciting protective immunity against the AIDS virus by strategies that are safe for human use suggest that novel technologies may be needed to develop an HIV vaccine. Recent studies in small animal influenza models have indicated that DNA inoculation can induce potent protective anti-viral immunity. The simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkey has proven a powerful model system for assessing novel AIDS virus vaccine strategies. We will employ the SIVmac/rhesus monkey model to study the use of DNA vaccines for preventing HIV infection. Specifically, we will explore the: 1. Construction of SIVmac DNA vaccine vectors 2. Immunogenicity of these constructs 3. Novel DNA vaccine delivery systems 4. Combined modality immunization employing DNA vaccines 5. Protective immunity achieved through DNA vaccination

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Project (R01)
Project #
Application #
Study Section
AIDS and Related Research Study Section 1 (ARRA)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Beth Israel Deaconess Medical Center
United States
Zip Code
Subbramanian, Ramu A; Charini, William A; Kuroda, Marcelo J et al. (2006) Expansion after epitope peptide exposure in vitro predicts cytotoxic T lymphocyte epitope dominance hierarchy in lymphocytes of vaccinated mamu-a*01+ rhesus monkeys. AIDS Res Hum Retroviruses 22:445-52
Greenland, John R; Liu, Huining; Berry, David et al. (2005) Beta-amino ester polymers facilitate in vivo DNA transfection and adjuvant plasmid DNA immunization. Mol Ther 12:164-70
Zhang, Lei; Ribeiro, Ruy M; Mascola, John R et al. (2004) Effects of antibody on viral kinetics in simian/human immunodeficiency virus infection: implications for vaccination. J Virol 78:5520-2
Barouch, Dan H; Truitt, Diana M; Letvin, Norman L (2004) Expression kinetics of the interleukin-2/immunoglobulin (IL-2/Ig) plasmid cytokine adjuvant. Vaccine 22:3092-7
Seaman, Michael S; Peyerl, Fred W; Jackson, Shawn S et al. (2004) Subsets of memory cytotoxic T lymphocytes elicited by vaccination influence the efficiency of secondary expansion in vivo. J Virol 78:206-15
McKay, Paul F; Barouch, Dan H; Santra, Sampa et al. (2004) Recruitment of different subsets of antigen-presenting cells selectively modulates DNA vaccine-elicited CD4+ and CD8+ T lymphocyte responses. Eur J Immunol 34:1011-20
McKay, Paul F; Barouch, Dan H; Schmitz, Jorn E et al. (2003) Global dysfunction of CD4 T-lymphocyte cytokine expression in simian-human immunodeficiency virus/SIV-infected monkeys is prevented by vaccination. J Virol 77:4695-702
Barouch, Dan H; Kunstman, Jennifer; Glowczwskie, Jennifer et al. (2003) Viral escape from dominant simian immunodeficiency virus epitope-specific cytotoxic T lymphocytes in DNA-vaccinated rhesus monkeys. J Virol 77:7367-75
Barouch, Dan H; McKay, Paul F; Sumida, Shawn M et al. (2003) Plasmid chemokines and colony-stimulating factors enhance the immunogenicity of DNA priming-viral vector boosting human immunodeficiency virus type 1 vaccines. J Virol 77:8729-35
Baig, Jamal; Levy, Daniel B; McKay, Paul F et al. (2002) Elicitation of simian immunodeficiency virus-specific cytotoxic T lymphocytes in mucosal compartments of rhesus monkeys by systemic vaccination. J Virol 76:11484-90

Showing the most recent 10 out of 61 publications