Our goal is to define the role of a2?1 integrin in the immune regulation of tumor progression and metastasis, with specific interest on the interplay between c-met and the a2?1 integrin. We present exciting data that the a2?1 integrin not only augments the innate immune response to pathogens, but promotes progression and metastasis of virus-induced squamous carcinoma. During the previous funding period we identified C1q and the collectin family of proteins as novel ligands for the a2?1 integrin and demonstrated cross-talk between the a2?1 integrin and c-met that was required for mast cell activation and the secretion of IL-6. We hypothesize that a2?1 integrin-mediated interactions between immune cells and tumor cells regulate tumor progression and metastasis. Inflammation, mast cells, T cells and immune complexes are required for tumor progression in the transgenic mouse model of epithelial carcinogenesis in which the human papillomavirus type 16 (HPV16) early region genes are expressed in basal keratinocytes. In this model, invasive squamous cell carcinoma occurs in 50% of wild type mice. We generated K14-HPV16/WT and HPV16/a2-null mice. In HPV16/a2-null mice, tumor latency and tumor growth are not altered in mice lacking the a2?1 integrin, but tumor burden and the number of animals with lymph node metastasis is markedly decreased in a2-null mice compared to wild type mice, suggesting that the a2?1 integrin promotes inflammation and tumor progression. We hypothesize that a2?1 integrin via interactions with c-met and stimulation of the immune system promotes epithelial carcinogenesis. We propose four aims using a combination of mouse models, human tumor samples, and in vitro cell biological and molecular techniques:
SPECIFIC AIM 1 : Determine the mechanism by which a2?1 integrin expression promotes tumor progression. FOCUS - a2?1 integrin and K14-HPV16 epithelial carcinogenesis.
SPECIFIC AIM 2 : Define cross-talk between the a2?1 integrin and HGF/c-met in tumor progression and metastasis. FOCUS - a2?1 integrin and HGF/c-met crosstalk.
SPECIFIC AIM 3 : Define the a2?1 integrin-dependent regulation of neoangiogenesis and lymphangiogenesis. FOCUS - Blood and lymphatic endothelial cells.
SPECIFIC AIM 4 : Define the functional and prognostic relevance of inflammation, mast cell infiltration, a2?1 integrin and c-met expression and the angiogenic phenotype in HPV-positive (+) and HPV-negative (-) squamous cell carcinoma of the head and neck (HNSCC). FOCUS - Squamous cell carcinoma of the head and neck.

Public Health Relevance

Tumor progression and metastasis are regulated by the host microenvironment including cells of the immune system. The goal of this proposal is to study a cell adhesion receptor, the a2?1 integrin that regulates cancer progression and metastasis.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA098027-09
Application #
8074980
Study Section
Tumor Microenvironment Study Section (TME)
Program Officer
Howcroft, Thomas K
Project Start
2003-01-01
Project End
2014-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
9
Fiscal Year
2011
Total Cost
$328,360
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Pathology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Madamanchi, Aasakiran; Zijlstra, Andries; Zutter, Mary M (2014) Flipping the switch: integrin switching provides metastatic competence. Sci Signal 7:pe9
Madamanchi, Aasakiran; Capozzi, Megan; Geng, Ling et al. (2014) Mitigation of oxygen-induced retinopathy in ?2?1 integrin-deficient mice. Invest Ophthalmol Vis Sci 55:4338-47
Tran, Thuy; Barlow, Brittney; O'Rear, Lynda et al. (2011) Loss of the ?2?1 integrin alters human papilloma virus-induced squamous carcinoma progression in vivo and in vitro. PLoS One 6:e26858
Ramirez, Norma E; Zhang, Zhonghua; Madamanchi, Aasakiran et al. (2011) The ???? integrin is a metastasis suppressor in mouse models and human cancer. J Clin Invest 121:226-37
McCall-Culbreath, Karissa D; Li, Zhengzhi; Zhang, Zhonghua et al. (2011) Selective, ?2?1 integrin-dependent secretion of il-6 by connective tissue mast cells. J Innate Immun 3:459-70
McCall-Culbreath, Karissa D; Li, Zhengzhi; Zutter, Mary M (2008) Crosstalk between the alpha2beta1 integrin and c-met/HGF-R regulates innate immunity. Blood 111:3562-70
Zhang, Zhonghua; Ramirez, Norma E; Yankeelov, Thomas E et al. (2008) alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner. Blood 111:1980-8
Zutter, Mary M; Edelson, Brian T (2007) The alpha2beta1 integrin: a novel collectin/C1q receptor. Immunobiology 212:343-53
Grenache, David G; Zhang, Zhonghua; Wells, Laura E et al. (2007) Wound healing in the alpha2beta1 integrin-deficient mouse: altered keratinocyte biology and dysregulated matrix metalloproteinase expression. J Invest Dermatol 127:455-66
Edelson, Brian T; Stricker, Thomas P; Li, Zhengzhi et al. (2006) Novel collectin/C1q receptor mediates mast cell activation and innate immunity. Blood 107:143-50

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