Osteoblast differentiation and bone formation are controlled by a wide range of extracellular signals including cell-extracellular matrix interaction, matrix stiffness, biomechanical force, hormones, growth factors and morphogens. To induce differentiation, each stimulus must initiate a signal that can be efficiently dispersed through the genome to induce global changes in gene expression necessary for the osteoblastogenesis while simultaneously suppressing other lineages. The following hypothesis is proposed to explain how this is accomplished: i) After exposure to an osteogenic stimulus, P-ERK1/2 translocates to the nucleus and binds chromatin of osteoblast target genes via specific docking sites on Runx2. ii) By phosphorylating RUNX2 on specific sites, kinases initiate chromatin remodeling and transcription;the overall level of RUNX2 phosphorylation is a reflection of the various extracellular stimuli to which the cell is exposed. iii) Conversely, kinases may phosphorylate other lineage-specific transcription factors on chromatin to suppress non-osteogenic lineages such as adipocytes. This hypothesis will be tested by achieving the following aims: 1. Evaluate the role of MAP kinase phosphorylation of RUNX2 and PPAR? in the reciprocal control of osteogenesis and adipogenesis. 2. Evaluate the role of RUNX2 phosphorylation by ERK1/2 in the control of fluid flow shear stress (FFSS)-induced osteoblast gene expression. 3. Determine the role of Runx2 S301,S319 phosphorylation in skeletal development and remodeling. Experiments include detailed epigenetic analyses of chromatin changes accompanying osteoblast versus adipocyte differentiation as well as genomic analysis using chromatin immunoprecipitation and next generation sequencing. These studies will test a new model for global control of gene expression that may explain how osteogenic stimuli, including extracellular matrix signals and mechanical loading, can reprogram the genome to stimulate osteoblast differentiation while suppressing other lineages such as adipocytes. Once this model is understood, it will be possible to manipulate osteogenic signals to optimize bone formation during remodeling and regeneration. Furthermore, this work will test the validity of using RUNX2 phosphorylation-site specific antibodies to monitor activation of this factor under different physiological conditions. This may have applications for diagnosis of osteoporosis and other bone disorders, such as osteoarthritis and certain cancers.
To successfully treat diseases like osteoporosis and osteoarthritis as well as regenerate bone, it is essential that we understand basic mechanisms controlling bone formation. This project will test a new hypothesis to explain how genes necessary for formation of osteoblasts, the bone-forming cells, become activated by anabolic signals such as mechanical loading and hormone/growth factor treatment. The central concept to be explored is that anabolic signals activate nuclear protein kinases, enzymes that add a phosphate group to transcription factors to either stimulate or inhibit their activity. Transcriptin factors subsequently regulate the activity of specific genes. We propose that protein kinases stimulate transcription factors necessary for osteoblast formation while inhibiting factors involved in fat cell formation. In this way, bone formation is favored. If correct, our hypothesis provides a new route for stimulating bone formation through pharmacological manipulation of protein kinase activity.
|Lino, Marsel; Wan, Mark H; Rocca, Antonio S et al. (2018) Diabetic Vascular Calcification Mediated by the Collagen Receptor Discoidin Domain Receptor 1 via the Phosphoinositide 3-Kinase/Akt/Runt-Related Transcription Factor 2 Signaling Axis. Arterioscler Thromb Vasc Biol 38:1878-1889|
|Ge, Chunxi; Zhao, Guisheng; Li, BinBin et al. (2018) Genetic inhibition of PPAR? S112 phosphorylation reduces bone formation and stimulates marrow adipogenesis. Bone 107:1-9|
|Ge, C; Mohamed, F; Binrayes, A et al. (2018) Selective Role of Discoidin Domain Receptor 2 in Murine Temporomandibular Joint Development and Aging. J Dent Res 97:321-328|
|Franceschi, Renny T; Ge, Chunxi (2017) Control of the Osteoblast Lineage by Mitogen-Activated Protein Kinase Signaling. Curr Mol Biol Rep 3:122-132|
|Li, Yan; Ge, Chunxi; Franceschi, Renny T (2017) MAP Kinase-Dependent RUNX2 Phosphorylation Is Necessary for Epigenetic Modification of Chromatin During Osteoblast Differentiation. J Cell Physiol 232:2427-2435|
|Mohamed, Fatma F; Franceschi, Renny T (2017) Skeletal Stem Cells: Origins, Functions and Uncertainties. Curr Mol Biol Rep 3:236-246|
|Gonzalez, Maria E; Martin, Emily E; Anwar, Talha et al. (2017) Mesenchymal Stem Cell-Induced DDR2 Mediates Stromal-Breast Cancer Interactions and Metastasis Growth. Cell Rep 18:1215-1228|
|Ge, Chunxi; Cawthorn, William P; Li, Yan et al. (2016) Reciprocal Control of Osteogenic and Adipogenic Differentiation by ERK/MAP Kinase Phosphorylation of Runx2 and PPAR? Transcription Factors. J Cell Physiol 231:587-96|
|Ge, C; Zhao, G; Li, Y et al. (2016) Role of Runx2 phosphorylation in prostate cancer and association with metastatic disease. Oncogene 35:366-76|
|Ge, Chunxi; Wang, Zhengyan; Zhao, Guisheng et al. (2016) Discoidin Receptor 2 Controls Bone Formation and Marrow Adipogenesis. J Bone Miner Res 31:2193-2203|
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