We try to understand mechanism and physiological significance of carbohydrate-protein interactions in animals. A major emphasis will be place on hepatic sugar binding proteins. we will examine the molecular binding mechanism of rabbit hepatic Gal/GalNAc-binding protein, by interacting a series of neoglycoproteins having different sugars and aglycons with the binding protein. Affinity labelling with synthetic glycoside will be attemtped. Additionally, lactoperoxidase modified with Gal/GalNAc derivatives, a good ligand for the binding protein, will be used for selective iodination of the binding protein. Comparative biochemistry of hepatic sugar-binding proteins will be studied. Sugar-binding specificity of chicken liver non-parenchymal cells will be examined. Sugar-binding specificity from livers of lower vertebrates will be compared. Sugar-binding proteins from these animals will be isolated, characterized and comparedwith the known hepatic sugar-binding proteins. We will also study sugar-binding proteins in animal sera with respect to specificity, molecular properties, sand physiological behavior. The clearance of immunological complex by carbohydrate-mediated receptors will be studied in depth using monoclonal antibodies, well-defined antigens, and mammalian hepatocytes and alveolar macrophages. We will also determine when the hepatic GlcNAc-binding activity is expressed during the developmental stages of chick embryo and juvenile chicken.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK009970-21
Application #
3224645
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1976-12-01
Project End
1986-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
21
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Arts and Sciences
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
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Betenbaugh, Michael J; Tomiya, Noboru; Narang, Someet et al. (2004) Biosynthesis of human-type N-glycans in heterologous systems. Curr Opin Struct Biol 14:601-6
Lee, Y C (2001) Fluorometric determination of EDTA and EGTA using terbium-salicylate complex. Anal Biochem 293:120-3