This grant seeks to better understand the structure and function of protein tyrosine phosphatases (PTP), a superfamily of enzymes playing important roles in signal transduction. Our laboratory has identified a PTP-associated adapter protein in Drosophilia melanogaster known as Dock. Dock plays an essential role in axon guidance in the fly. We have isolated five Dock-associated proteins. We plan to clone and characterize these cDNAs and corresponding proteins in order to better understand their interactions with Dock, the PTP, as well as their roles in axonal guidance. Our goals also include the use of a novel strategy to identify substrates for two S. cerevisae phosphatases, ACR2 and YG4E. We anticipate that our strategies may be extended to understand the functions of other yeast phosphatases. Finally, our laboratory has solved the structure for the first phospholipase D supergene family member. The PLD supergene family members play key roles in signal transduction, vesicle traffic, mitosis, and bacterial pathogenesis. We plan to extend our structural and functional studies to other members of the PLD supergene family.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK018849-30
Application #
6634848
Study Section
Biochemistry Study Section (BIO)
Program Officer
Blondel, Olivier
Project Start
1991-10-01
Project End
2005-05-31
Budget Start
2003-07-01
Budget End
2005-05-31
Support Year
30
Fiscal Year
2003
Total Cost
$482,368
Indirect Cost
Name
University of California San Diego
Department
Pharmacology
Type
Schools of Medicine
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
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