The major goal of the proposal is to understand the molecular biology and replication strategies of the rotaviruses. The three specific aims (taken from the proposal) are: (1) To understand the protein-protein interactions between the viral capsid proteins and the nonstructural glycoprotein NSP4. The relevant domains will be identified by deletion and mutational analysis. The studies are to provide a structural foundation for the understanding of morphogenesis, particle stability and antigenicity as well as virus binding and entry into cells. The second specific aim is to understand the molecular mechanisms and domains responsible for the different properties associated with the nonstructural glycoprotein NSP4. The third specific aim is to understand the morphogenetic process in which particles bud through the membrane of the endoplasmic reticulum and acquire the outer capsid proteins VP4 and VP7. The sequential steps by which single- shelled particles become enveloped and mature into double-shelled particles by the addition of VP4 and VP7 will be determined.
| Kavanagh, Owen V; Ajami, Nadim J; Cheng, Elly et al. (2010) Rotavirus enterotoxin NSP4 has mucosal adjuvant properties. Vaccine 28:3106-11 |
| Greenberg, Harry B; Estes, Mary K (2009) Rotaviruses: from pathogenesis to vaccination. Gastroenterology 136:1939-51 |
| Li, Zongli; Baker, Matthew L; Jiang, Wen et al. (2009) Rotavirus architecture at subnanometer resolution. J Virol 83:1754-66 |
| Gilger, Mark A; El-Serag, Hashem B; Gold, Benjamin D et al. (2008) Prevalence of endoscopic findings of erosive esophagitis in children: a population-based study. J Pediatr Gastroenterol Nutr 47:141-6 |
| Berkova, Zuzana; Crawford, Sue E; Blutt, Sarah E et al. (2007) Expression of rotavirus NSP4 alters the actin network organization through the actin remodeling protein cofilin. J Virol 81:3545-53 |
| Berkova, Z; Crawford, S E; Trugnan, G et al. (2006) Rotavirus NSP4 induces a novel vesicular compartment regulated by calcium and associated with viroplasms. J Virol 80:6061-71 |
| El-Serag, Hashem B; Gilger, Mark A; Shub, Mitchell D et al. (2006) The prevalence of suspected Barrett's esophagus in children and adolescents: a multicenter endoscopic study. Gastrointest Endosc 64:671-5 |
| Gilger, Mark A; Gold, Benjamin D (2005) Pediatric endoscopy: new information from the PEDS-CORI project. Curr Gastroenterol Rep 7:234-9 |
