The objectives of this proposal are to investigate mechanisms whereby positive and negative regulatory elements in the ovalbumin promoter direct appropriate temporal and tissue-specific expression. The three Specific Aims are: 1) investigate the hormone response unit in the Ov gene, 2) define the role of the negative regulatory element in positive and negative regulation of the Ov gene, and 3) investigate estrogen signal transduction cascades in the developing oviduct.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK040082-16
Application #
6634943
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Margolis, Ronald N
Project Start
1988-05-01
Project End
2005-06-30
Budget Start
2003-07-01
Budget End
2005-06-30
Support Year
16
Fiscal Year
2003
Total Cost
$238,623
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Biochemistry
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Hurt, Elaine M; Saykally, Jessica N; Anose, Bynthia M et al. (2008) Expression of the ZEB1 (deltaEF1) transcription factor in human: additional insights. Mol Cell Biochem 318:89-99
Dougherty, Dawne C; Sanders, Michel M (2005) Comparison of the responsiveness of the pGL3 and pGL4 luciferase reporter vectors to steroid hormones. Biotechniques 39:203-7
Dougherty, Dawne C; Sanders, Michel M (2005) Estrogen action: revitalization of the chick oviduct model. Trends Endocrinol Metab 16:414-9
Dillner, Naomi B; Sanders, Michel M (2004) Transcriptional activation by the zinc-finger homeodomain protein delta EF1 in estrogen signaling cascades. DNA Cell Biol 23:25-34
Monroe, David G; Berger, Ryan R; Sanders, Michel M (2002) Tissue-protective effects of estrogen involve regulation of caspase gene expression. Mol Endocrinol 16:1322-31
Dillner, Naomi B; Sanders, Michel M (2002) Upstream stimulatory factor (USF) is recruited into a steroid hormone-triggered regulatory circuit by the estrogen-inducible transcription factor delta EF1. J Biol Chem 277:33890-4
Monroe, D G; Jin, D F; Sanders, M M (2000) Estrogen opposes the apoptotic effects of bone morphogenetic protein 7 on tissue remodeling. Mol Cell Biol 20:4626-34
Berger, R R; Sanders, M M (2000) Estrogen modulates HNF-3beta mRNA levels in the developing chick oviduct. DNA Cell Biol 19:103-12
Sensenbaugh, K R; Sanders, M M (1999) Multiple promoter elements including a novel repressor site modulate expression of the chick ovalbumin gene. DNA Cell Biol 18:147-56
Chamberlain, E M; Sanders, M M (1999) Identification of the novel player deltaEF1 in estrogen transcriptional cascades. Mol Cell Biol 19:3600-6

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