Dietary protein intake alters energy expenditure and remodels adipose tissue, but the cellular mechanisms mediating these effects are largely undescribed. We recently discovered that the hormone FGF21 is robustly increased by dietary protein restriction and that mice lacking FGF21 fail to exhibit the expected changes in growth, energy expenditure or glucose homeostasis in response to dietary restriction. In addition, selective deletion of the FGF21 co-receptor beta-Klotho (Klb) from the brain also blocks the metabolic response to protein restriction. This discovery has redefined FGF21's physiological role by suggesting that it acts directly in the brain to coordinate the adaptive, homeostatic response to protein restriction. This project extends these data by 1) Identifying the FGF21-dependent neurobiological mechanisms that mediate changes in energy expenditure during protein restriction, and 2) Determining the mechanism through which protein restriction, via brain FGF21 signaling, remodels adipose tissue and thereby influences energy expenditure and metabolic health. This project will define the mechanism through which animals sense and respond to protein restriction while also delineating the mechanisms through which FGF21 acts in the brain to stimulate energy expenditure.

Public Health Relevance

This project seeks to identify novel mechanisms which mediate the effects of dietary protein intake on energy expenditure. There is ample evidence that dietary protein impacts metabolism and health, and our lab recently discovered that the hormone FGF21 is essential for metabolic responses to protein restriction. This project seeks to define how FGF21 signaling within the brain alters metabolism and the extent to which the FGF21- dependent remodeling of adipose tissue contributes to the beneficial metabolic effects of dietary protein restriction. It is expected that this work will uncover fundamentally new pathways linking diet to metabolic health.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK121370-01A1
Application #
9973291
Study Section
Integrative Physiology of Obesity and Diabetes Study Section (IPOD)
Program Officer
Cooke, Bradley Monroe
Project Start
2020-05-15
Project End
2024-02-29
Budget Start
2020-05-15
Budget End
2021-02-28
Support Year
1
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Lsu Pennington Biomedical Research Center
Department
Type
Organized Research Units
DUNS #
611012324
City
Baton Rouge
State
LA
Country
United States
Zip Code
70808