Goal: Develop an array of capture molecules for pathogens, intended for use in a rapid screening format, that includes the following: 1. specific capture molecules for different species of microorganisms or their toxic products; 2. semi-selective capture molecules that identify pathogens based on mechanisms involved in the process; of pathogenicity; 3. indicators of potential susceptibility to antibiotics. The first step in this process is to modify candidates for the array and immobilize them in a sensor for rapid characterization. The candidates will include antibodies, oligosaccharides, adhesions, siderophores, combinatorial peptides which bind membrane proteins on the pathogen, and antibiotics. The candidates will be exposed to a broad spectrum of pathogens and toxins to determine both specificity and binding affinity. The elements of the array will be selected to provide the widest amount of information possible: species if possible, mechanism of infection or pathogenicity, and antibiotic susceptibility. The array elements will then be transferred to a corporate partner with demonstrated capability of producing thousands of sensor arrays for clinical use.
| Taitt, Chris R; North, Stella H; Kulagina, Nadezhda V (2009) Antimicrobial peptide arrays for detection of inactivated biothreat agents. Methods Mol Biol 570:233-55 |
| Kulagina, Nadezhda V; Shaffer, Kara M; Ligler, Frances S et al. (2007) Antimicrobial peptides as new recognition molecules for screening challenging species. Sens Actuators B Chem 121:150-157 |
| Ligler, Frances S; Sapsford, Kim E; Golden, Joel P et al. (2007) The array biosensor: portable, automated systems. Anal Sci 23:5-10 |
| Johnson-White, Brandy; Buquo, Lauren; Zeinali, Mazyar et al. (2006) Prevention of nonspecific bacterial cell adhesion in immunoassays by use of cranberry juice. Anal Chem 78:853-7 |
| Ngundi, Miriam M; Taitt, Chris R; Ligler, Frances S (2006) Simultaneous determination of kinetic parameters for the binding of cholera toxin to immobilized sialic acid and monoclonal antibody using an array biosensor. Biosens Bioelectron 22:124-30 |
| Moreno-Bondi, Maria C; Taitt, Chris Rowe; Shriver-Lake, Lisa C et al. (2006) Multiplexed measurement of serum antibodies using an array biosensor. Biosens Bioelectron 21:1880-6 |
| Ngundi, Miriam M; Taitt, Chris R; McMurry, Scott A et al. (2006) Detection of bacterial toxins with monosaccharide arrays. Biosens Bioelectron 21:1195-201 |
| Lassman, Michael E; Kulagina, Nadia; Taitt, Chris Rowe (2004) Fragmentation of biotinylated cyclic peptides. Rapid Commun Mass Spectrom 18:1277-85 |
