Goal: Develop an array of capture molecules for pathogens, intended for use in a rapid screening format, that includes the following: 1. specific capture molecules for different species of microorganisms or their toxic products; 2. semi-selective capture molecules that identify pathogens based on mechanisms involved in the process; of pathogenicity; 3. indicators of potential susceptibility to antibiotics. The first step in this process is to modify candidates for the array and immobilize them in a sensor for rapid characterization. The candidates will include antibodies, oligosaccharides, adhesions, siderophores, combinatorial peptides which bind membrane proteins on the pathogen, and antibiotics. The candidates will be exposed to a broad spectrum of pathogens and toxins to determine both specificity and binding affinity. The elements of the array will be selected to provide the widest amount of information possible: species if possible, mechanism of infection or pathogenicity, and antibiotic susceptibility. The array elements will then be transferred to a corporate partner with demonstrated capability of producing thousands of sensor arrays for clinical use.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB000680-03
Application #
6889244
Study Section
Special Emphasis Panel (ZRG1-SSS-F (02))
Program Officer
Korte, Brenda
Project Start
2003-05-01
Project End
2006-09-30
Budget Start
2005-05-01
Budget End
2006-09-30
Support Year
3
Fiscal Year
2005
Total Cost
$591,929
Indirect Cost
Name
U.S. Naval Research Laboratory
Department
Type
DUNS #
020060658
City
Washington
State
DC
Country
United States
Zip Code
20375
Taitt, Chris R; North, Stella H; Kulagina, Nadezhda V (2009) Antimicrobial peptide arrays for detection of inactivated biothreat agents. Methods Mol Biol 570:233-55
Kulagina, Nadezhda V; Shaffer, Kara M; Ligler, Frances S et al. (2007) Antimicrobial peptides as new recognition molecules for screening challenging species. Sens Actuators B Chem 121:150-157
Ligler, Frances S; Sapsford, Kim E; Golden, Joel P et al. (2007) The array biosensor: portable, automated systems. Anal Sci 23:5-10
Ngundi, Miriam M; Taitt, Chris R; McMurry, Scott A et al. (2006) Detection of bacterial toxins with monosaccharide arrays. Biosens Bioelectron 21:1195-201
Johnson-White, Brandy; Buquo, Lauren; Zeinali, Mazyar et al. (2006) Prevention of nonspecific bacterial cell adhesion in immunoassays by use of cranberry juice. Anal Chem 78:853-7
Ngundi, Miriam M; Taitt, Chris R; Ligler, Frances S (2006) Simultaneous determination of kinetic parameters for the binding of cholera toxin to immobilized sialic acid and monoclonal antibody using an array biosensor. Biosens Bioelectron 22:124-30
Moreno-Bondi, Maria C; Taitt, Chris Rowe; Shriver-Lake, Lisa C et al. (2006) Multiplexed measurement of serum antibodies using an array biosensor. Biosens Bioelectron 21:1880-6
Lassman, Michael E; Kulagina, Nadia; Taitt, Chris Rowe (2004) Fragmentation of biotinylated cyclic peptides. Rapid Commun Mass Spectrom 18:1277-85