The primary goal of this project is to characterize the ocular zonule biochemically, ultrastructurally and immunologically, to provide tools for investigating diseases of this basic unit in the elastic tissue system. The glycoprotein composing the zonular fibers will be isolated and characterized in bovine and human eyes, to better define zonular microfibrillar protein. Polyclonal and monoclonal antibodies made to these purified proteins will be used in tissues for investigating abnormalities of the zonule and the ocular elastic tissues. These diseases result in zonular breakage and dislocation of the lens, but are frequently associated with disorders of ocular growth, especially myopia, and so have a broader significance. The dermal elastic system will also be studied in these diseases, as well as in the pseudo-exfoliative syndrome to determine whether the changes in the elastic micro- brillar system are generalized. The structural organization of the zonular fiber and its components will be studied by rotary shadowing and by immuno-electron microscopy using zonular and matrix antibodies. The cells involved in zonular fibrillogenesis will be investigated in fetal rabbit and bovine eyes by immunochemistry, and evidence sought for zonular receptors on the ciliary and lens epithelial cell surfaces. Adhesion proteins mediating zonular-basement membrane attachment will also be investigated immunologically. In collaborative work, zonular antibodies will be used to obtain cDNAs for sequencing zonular protein and for developing specific zonular probes.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
2R01EY001602-14
Application #
3256042
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1978-06-30
Project End
1991-06-29
Budget Start
1988-06-30
Budget End
1989-06-29
Support Year
14
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Upstate Medical University
Department
Type
Schools of Medicine
DUNS #
058889106
City
Syracuse
State
NY
Country
United States
Zip Code
13210
Famiglietti, Edward V; Stopa, Edward G; McGookin, Edward D et al. (2003) Immunocytochemical localization of vascular endothelial growth factor in neurons and glial cells of human retina. Brain Res 969:195-204
Aldave, A J; Eagle Jr, R C; Streeten, B W et al. (2001) Congenital corneal opacification in De Barsy syndrome. Arch Ophthalmol 119:285-8
Streeten, B W; Qi, Y; Klintworth, G K et al. (1999) Immunolocalization of beta ig-h3 protein in 5q31-linked corneal dystrophies and normal corneas. Arch Ophthalmol 117:67-75
Qi, Y; Streeten, B W; Wallace, R N (1997) HNK-1 epitope in the lens-ciliary zonular region in normal and pseudoexfoliative eyes. Immunohistochemistry and ultrastructure. Arch Ophthalmol 115:637-44
Konstas, A G; Ritch, R; Bufidis, T et al. (1997) Exfoliation syndrome in a 17-year-old girl. Arch Ophthalmol 115:1063-7
Netland, P A; Ye, H; Streeten, B W et al. (1995) Elastosis of the lamina cribrosa in pseudoexfoliation syndrome with glaucoma. Ophthalmology 102:878-86
Horrigan, S K; Rich, C B; Streeten, B W et al. (1992) Characterization of an associated microfibril protein through recombinant DNA techniques. J Biol Chem 267:10087-95
Li, Z Y; Wallace, R N; Streeten, B W et al. (1991) Elastic fiber components and protease inhibitors in pinguecula. Invest Ophthalmol Vis Sci 32:1573-85
Wallace, R N; Streeten, B W; Hanna, R B (1991) Rotary shadowing of elastic system microfibrils in the ocular zonule, vitreous, and ligamentum nuchae. Curr Eye Res 10:99-109
Stewart 3rd, D H; Streeten, B W; Brockhurst, R J et al. (1991) Abnormal scleral collagen in nanophthalmos. An ultrastructural study. Arch Ophthalmol 109:1017-25

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